Detailed information of TA system TA220530

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 205498..206053
• Replicon: plasmid pDSM110093_b
• Accession: NZ_CP085169
• Organism: Sulfitobacter sp. DSM 110093

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: DSM110093_RS19125
• Protein ID: WP_243267800.1
• Coordinates: 205742..206053 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: DSM110093_RS19120
• Protein ID: WP_243267799.1
• Coordinates: 205498..205740 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DSM110093_RS19100 (DSM110093_03890)	201327..202649	+	1323	WP_243267796.1	glutamine synthetase family protein	-
DSM110093_RS19105 (DSM110093_03892)	202772..203038	-	267	WP_243267797.1	Txe/YoeB family addiction module toxin	-
DSM110093_RS19110 (DSM110093_03893)	203035..203286	-	252	WP_243267798.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
DSM110093_RS19115 (DSM110093_03894)	203508..204857	-	1350	WP_243263448.1	Fic family protein	-
DSM110093_RS19120 (DSM110093_03895)	205498..205740	+	243	WP_243267799.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
DSM110093_RS19125 (DSM110093_03896)	205742..206053	+	312	WP_243267800.1	endoribonuclease MazF	Toxin
DSM110093_RS19130 (DSM110093_03897)	206097..206444	-	348	WP_243267801.1	type II toxin-antitoxin system HicB family antitoxin	-
DSM110093_RS19135 (DSM110093_03898)	206441..206695	-	255	WP_243267802.1	type II toxin-antitoxin system HicA family toxin	-
DSM110093_RS19140 (DSM110093_03900)	206846..207625	-	780	WP_243267803.1	IS30 family transposase	-
DSM110093_RS19145 (DSM110093_03901)	207758..208057	-	300	WP_243268008.1	CcdB family protein	-
DSM110093_RS19150 (DSM110093_03902)	208057..208287	-	231	WP_243268009.1	type II toxin-antitoxin system CcdA family antitoxin	-
DSM110093_RS19155	208498..208679	+	182	Protein_171	IS6 family transposase	-
DSM110093_RS19160 (DSM110093_03904)	208778..209494	-	717	WP_243268010.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..273772	273772

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-95)

Antitoxin

• Antitoxin PemI-like (1-79)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11295.38 Da
• Isoelectric Point: 10.2592

>T220530 WP_243267800.1 NZ_CP085169:205742-206053 [Sulfitobacter sp. DSM 110093]
MTYIPEAGDIVWLQFNPQAGHRPALVLSPAKYNKIGLMLCCPMTTKVKGYPFEVVIKGSPISAALADQVESLDWKVRRAK
RKGQVTISELAEVRAKSVALIKG

Nucleotide

• Length: 312 bp

>T220530 NZ_CP117163:c33851-32625 [Sphingobium sp. AntQ-1]
ATGAGCGCACGCCGTTTGACCGTGTGGTGGGACGGGCGGATCGCGGGCTGTCTGGCGCAGGATCAGCATGGGGATCTATC
CTTTGCCTATGATGCGGACTGGCTGGATCGCTCCGATGCACGCCCGCTGTCTCACTCGCTGCCGTTGCAACCGGATATTT
TCGATCGGCGCGCCTGTCGGCCCTTCTTCGGCGGACTCTTGCCCGAAGCGGACCAGCGCAGCGGCGTAGCGGGCGCGCTC
GGCGTGTCGGCCGCCAATGAGTTCGCGCTGCTCGACCGGTTGGGCGGCGATGTTGCGGGCGCGCTGACATTGCTGCCGGA
GGGCGAGATACCCCCCTCCCCTGCCATCTTTGCCGATCCAGCCTTGCCCCTGTCAGACGCCGCTTTTGCCGCCATCCTTG
ATCGCTTGCCGCACCAGCCCATGCTGGCGGGGGAAGCGGGCCTGCGCATGTCGCTGGCGGGCGCGCAGGCGAAGCTGCCG
GTGATCCTTGTCGATGGTCGCCCCGCCCTCCCCCGCCCCGGCGAGGCGACGACGCATATCGTCAAGCCGGAGATTCCTCG
CTTCGCCGGATCGGTCGCCAATGAAGCTTGGTGCATGGCGCTGGCCCGCACGGTCGGACTGGACGTAGCCGAGGCGGTCG
CGTGCGAGGCAGACGGGCGACCCTATCTGCTCGTCACCCGCTATGATCGGGCGCAGCGCGATGGCGTGATGGTCCGCCTG
CACCAGGAGGATGCGTGCCAGGCTTTAGGTATTCCGTCCGAACGCAAATATGCAGCAGAAGGCGGGCCAGCCTTTCGGGA
TATGTTTGCGCTCACCCGCGCCTATGTCCGGCAACCGGCGGTCGATGTCATCAGGCTGGTCGATGCGGCGATCTTCAACC
TCGCGATCGGCAATGCTGACGCACATGGGAAGAATTTCTCCTTCCTGCTCGATGGCGATGGGCCTCGGCTTGCGCCGCTC
TATGATCTGCTTTCAACGCGGGCGTGGCCGGAATTGAGCGCGCGGCAGGCGATGCGGGTCGGACGGGCCGGCATGATCGA
GGAACTGGACCAGGATGCCTGGCTGCGCTTTGCGAGCGATGCGGGGATCACCCTGCCCTTCCTCCGTCGCCGGATCATCA
CGATGACGGACGCAATCATGTCGGTTGTCGATGAGGCGCGCACATCGTCGGCGATTGCTGTGCAGACCGGATTGCGAGCT
GCGATGTTGCGTCAGTCCCTGGATTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8685.92 Da
• Isoelectric Point: 5.0022

>AT220530 WP_243267799.1 NZ_CP085169:205498-205740 [Sulfitobacter sp. DSM 110093]
MRVIVKKWGNSASVRIPAAIMQAAKLSLDTPVDVREENGRIIIEPDRSPEFLLDDLLNGITSDNAHASIKTGGPLGQETF

Nucleotide

• Length: 243 bp

>AT220530 NZ_CP117163:c34066-33848 [Sphingobium sp. AntQ-1]
ATGATCAAATTGACCTCCACACAGGCGCTCGGTGCTGCTGTCCGTCTCGCGCGCAAACGTGCGGGCCTGCGTCAGGATGA
ACTAGCCGGGGCGGCGGGTGTCGGCATTCGTTTCATCGTCGAACTGGAGGCAGGCAAGCCTACGCTGCAACTGGGCAAGG
CACTGGCGGTGCTGGCGGCGCTGGGCCACAGCCTGCATATCGATACCGAAACGCCATGA