Detailed information of TA system TA220528

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 260568..261123
• Replicon: plasmid pDSM110093_a
• Accession: NZ_CP085168
• Organism: Sulfitobacter sp. DSM 110093

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: DSM110093_RS17895
• Protein ID: WP_243267800.1
• Coordinates: 260812..261123 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: DSM110093_RS17890
• Protein ID: WP_243267799.1
• Coordinates: 260568..260810 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DSM110093_RS17870 (DSM110093_03629)	256397..257719	+	1323	WP_243267796.1	glutamine synthetase family protein	-
DSM110093_RS17875 (DSM110093_03631)	257842..258108	-	267	WP_243267797.1	Txe/YoeB family addiction module toxin	-
DSM110093_RS17880 (DSM110093_03632)	258105..258356	-	252	WP_243267798.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
DSM110093_RS17885 (DSM110093_03633)	258578..259927	-	1350	WP_243263448.1	Fic family protein	-
DSM110093_RS17890 (DSM110093_03634)	260568..260810	+	243	WP_243267799.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
DSM110093_RS17895 (DSM110093_03635)	260812..261123	+	312	WP_243267800.1	endoribonuclease MazF	Toxin
DSM110093_RS17900 (DSM110093_03636)	261167..261514	-	348	WP_243267801.1	type II toxin-antitoxin system HicB family antitoxin	-
DSM110093_RS17905 (DSM110093_03637)	261511..261765	-	255	WP_243267802.1	type II toxin-antitoxin system HicA family toxin	-
DSM110093_RS17910 (DSM110093_03639)	261916..262695	-	780	WP_243267803.1	IS30 family transposase	-
DSM110093_RS17915	262904..263032	-	129	Protein_223	transposase	-
DSM110093_RS17920	263104..263337	+	234	Protein_224	transposase	-
DSM110093_RS17925 (DSM110093_03641)	263341..264492	-	1152	WP_243267804.1	sulfotransferase family 2 domain-containing protein	-
DSM110093_RS17930 (DSM110093_03642)	265264..266046	+	783	WP_243267805.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..336544	336544

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-95)

Antitoxin

• Antitoxin PemI-like (1-79)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11295.38 Da
• Isoelectric Point: 10.2592

>T220528 WP_243267800.1 NZ_CP085168:260812-261123 [Sulfitobacter sp. DSM 110093]
MTYIPEAGDIVWLQFNPQAGHRPALVLSPAKYNKIGLMLCCPMTTKVKGYPFEVVIKGSPISAALADQVESLDWKVRRAK
RKGQVTISELAEVRAKSVALIKG

Nucleotide

• Length: 312 bp

>T220528 NZ_CP117159:248161-248448 [Sphingobium sp. AntQ-1]
ATGAGGCTGCGCTGGACGGCCGATGCCGTCAGGGATCGTGACGCCATTTACGACTATGTCGAAGCGCGCAGCCCGCGCGC
GGCGATCAGGCTCGACACGCTCTTTTCGCAAAGAGCAGCCGACCTGTCCCGGCTCCCTGCCATGGGCCGGCCTGGCCGGG
TGCCGGGAACGCGCGAACTAATCGCGCATCGCAACTATATTCTGGTCTATGACCTGACCGATACGGACGTGCGCATCCTG
CGCCTGCTTCACGCCGCGCGCCTGTGGCCGCCACAAGAGCGGCGCTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8685.92 Da
• Isoelectric Point: 5.0022

>AT220528 WP_243267799.1 NZ_CP085168:260568-260810 [Sulfitobacter sp. DSM 110093]
MRVIVKKWGNSASVRIPAAIMQAAKLSLDTPVDVREENGRIIIEPDRSPEFLLDDLLNGITSDNAHASIKTGGPLGQETF

Nucleotide

• Length: 243 bp

>AT220528 NZ_CP117159:247877-248164 [Sphingobium sp. AntQ-1]
ATGAATGAGGCGACCTTCACCTTTCGCGTGGATCAGGAACTCAAGGGCCGCTTCGCCCGCGCGGCCAAGGCGCAGGATCG
CACCGGCGCGCAATTGTTGCGCGATTTCATGCGCGATGTCGTACTGCGCGAGGAACAGGCCGAAAGCCATGACGCCTGGT
TCCGTCGTCAGGTCGACGCCGGACGCGCATCCGCGCAGGCAGGCCACCTCATCGCCGGCGATGAAGTCGAGGCGCATTTC
GCCGCACGACGCGCCGCCACGCTGCGGACATCAGACGGCGAGCGATGA