Detailed information of TA system TA220525

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 31701..32166
• Replicon: plasmid pDSM109990_e
• Accession: NZ_CP085149
• Organism: Sulfitobacter dubius strain DSM 109990

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: DSM109990_RS20225
• Protein ID: WP_174861618.1
• Coordinates: 31701..31964 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A073IVW0
• Locus tag: DSM109990_RS20230
• Protein ID: WP_037931637.1
• Coordinates: 31951..32166 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DSM109990_RS20190 (DSM109990_04109)	27468..27668	-	201	Protein_27	DUF4158 domain-containing protein	-
DSM109990_RS20195 (DSM109990_04110)	27763..28371	+	609	WP_036742596.1	recombinase family protein	-
DSM109990_RS20200	28403..28570	+	168	Protein_29	helix-turn-helix domain-containing protein	-
DSM109990_RS20205 (DSM109990_04111)	28735..29670	-	936	WP_231582226.1	hypothetical protein	-
DSM109990_RS20210 (DSM109990_04112)	29611..30477	+	867	WP_047998256.1	LysR family transcriptional regulator	-
DSM109990_RS20215 (DSM109990_04113)	30734..31363	+	630	WP_243263987.1	tyrosine-type recombinase/integrase	-
DSM109990_RS20220 (DSM109990_04114)	31369..31620	+	252	WP_174861619.1	WGR domain-containing protein	-
DSM109990_RS20225 (DSM109990_04115)	31701..31964	+	264	WP_174861618.1	BrnT family toxin	Toxin
DSM109990_RS20230 (DSM109990_04116)	31951..32166	+	216	WP_037931637.1	hypothetical protein	Antitoxin
DSM109990_RS20235 (DSM109990_04117)	32197..32913	-	717	WP_174861617.1	SprT family zinc-dependent metalloprotease	-
DSM109990_RS20240 (DSM109990_04118)	32910..36119	-	3210	WP_243263988.1	HsdR family type I site-specific deoxyribonuclease	-
DSM109990_RS20245 (DSM109990_04119)	36116..36955	-	840	WP_243263989.1	DUF3800 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..47721	47721

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-80)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10157.26 Da
• Isoelectric Point: 5.1298

>T220525 WP_174861618.1 NZ_CP085149:31701-31964 [Sulfitobacter dubius]
MKFEYDPDKSAANREKHGIDFDEAQALWNDPYLIEAPANVTDEPRFLAVGMIGARHWTAVYTYRSDRVRIISVRRARTQE
IDHYEGD

Nucleotide

• Length: 264 bp

>T220525 NZ_CP117054:c809259-808957 [Vibrio harveyi]
ATGGCTGAAGTAATCTGGACTGAACCAGCGTTATCTGACCTCAATGATATCGCTGAATACATCGCACTTGAAAATATCGT
AGCTGCAAAGCAGTTAGTACAAACGATCTTCTCCAAAGTCGAACGCCTACGAACTTTCCCAGAGTCAGGTCGTATTCCAC
CCGAGCTAGAGCATTTAAGTTATCGTGAAGTTGTCGTTAATCCATGTCGTGTTTTCTATAAACAAGACGGTGACAAAGTG
TTTATTCTGTTTGTTATGCGTGCAGAGAGAGATTTGCGTAAGTTCTTGTTGGGTAAGCAATAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8274.49 Da
• Isoelectric Point: 7.0141

>AT220525 WP_037931637.1 NZ_CP085149:31951-32166 [Sulfitobacter dubius]
MKATEFDERFEAGEDMSAHVDWTKARRLNVEAKRVNVDFPTWVVAGLDRQAQKLGITRQALIKMWIAERLE

Nucleotide

• Length: 216 bp

>AT220525 NZ_CP117054:c809504-809247 [Vibrio harveyi]
ATGAAAGTAGAACTAGTTACATCACTCAAACGTCAAGCAACTAAGATCCTCGCCGATCTCCACGATACCAAAGAGCCAGT
GTTAATTACTGAGCATGGTAAGCCATCCGCTTATCTCGTTGATGTTGATGACTACGAGTTTATGCAAAATCGTTTAGCTA
TTCTTGAGGGTATTGCACGAGGTGAGCGTGCACTAGCTGACGGTAAAATGGTAAGCCATGATGAAGCCAAGGACAAAATG
TCAAAATGGCTGAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A073IVW0