Detailed information of TA system TA220523

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ParE-CopA/ParE-RHH
• Location: 124020..124585
• Replicon: plasmid pDSM109990_a
• Accession: NZ_CP085145
• Organism: Sulfitobacter dubius strain DSM 109990

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: DSM109990_RS16835
• Protein ID: WP_243263507.1
• Coordinates: 124286..124585 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: copA
• Uniprot ID: -
• Locus tag: DSM109990_RS16830
• Protein ID: WP_243263506.1
• Coordinates: 124020..124298 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DSM109990_RS16810 (DSM109990_03410)	120142..121527	+	1386	WP_243263500.1	glycosyltransferase family 1 protein	-
DSM109990_RS16815 (DSM109990_03411)	121550..121813	+	264	WP_243263502.1	type II toxin-antitoxin system ParD family antitoxin	-
DSM109990_RS16820 (DSM109990_03412)	122018..122203	+	186	WP_243263503.1	hypothetical protein	-
DSM109990_RS16825 (DSM109990_03413)	122917..123546	-	630	WP_243263504.1	class I SAM-dependent methyltransferase	-
DSM109990_RS16830 (DSM109990_03414)	124020..124298	+	279	WP_243263506.1	CopG family ribbon-helix-helix protein	Antitoxin
DSM109990_RS16835 (DSM109990_03415)	124286..124585	+	300	WP_243263507.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DSM109990_RS16840 (DSM109990_03416)	124737..126119	-	1383	WP_243263509.1	IS1182 family transposase	-
DSM109990_RS16845 (DSM109990_03417)	126445..127404	+	960	WP_243263511.1	helix-turn-helix domain-containing protein	-
DSM109990_RS16850 (DSM109990_03418)	127534..129057	+	1524	WP_243263512.1	aldehyde dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..284454	284454
	-	flank	IS/Tn	-	-	124737..126119	1382

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-94)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-42)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11178.68 Da
• Isoelectric Point: 5.0878

>T220523 WP_243263507.1 NZ_CP085145:124286-124585 [Sulfitobacter dubius]
MPRLIWSPEALADVQRLYRFLAEKNPDAARRAASAIRDGMQIVADHPDVGRPVDDMEPEFQEWPISFGASGYVALYRLQQ
DVALVVAVRHQKEVGYQPS

Nucleotide

• Length: 300 bp

>T220523 NZ_CP117054:c780592-780290 [Vibrio harveyi]
ATGGCTGAAATAATCTGGACTGAGCCAGCGTTATCTGACCTCAACGATATCGCTGAATATATCGCACTTGAAAATATCGT
AGCTGCAAAGCAGTTAGTGCAAACGATCTTCTCCAAAGTCGAACGCCTGCAAACTTTCCCAGAGTCAGGTCGTATTCCAC
CCGAGCTAGAACATTTAAGTTATCGTGAAGTTGTCGTTAATCCATGTCGTGTTTTCTATAAACAAGACGGTGACAAAGTG
TTTATTCTGTTTGTTATGCGTGCAGAGAGAGATTTGCGTAAGTTCCTGTTGAGTAAGCAGTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10535.79 Da
• Isoelectric Point: 5.1499

>AT220523 WP_243263506.1 NZ_CP085145:124020-124298 [Sulfitobacter dubius]
MPTPVAVKLDEDVHSRMKVLAEARDRSPHWMMREAIAQYVEREEKREAFRQDGIRAWNAYQETGLHVTHAEADAWLAQLA
AGDDQEPPACHV

Nucleotide

• Length: 279 bp

>AT220523 NZ_CP117054:c780837-780580 [Vibrio harveyi]
ATGAAAGTAGAACTAGTTACATCACTTAAACGTCAAGCAACTAAGATCCTCGCCGATCTCCACGACACCAAAGAACCAGT
GTTAATTACTGAACATGGTAAGCCATCGGCATATCTGGTTGATGTTGATGATTACGAGTTTATGCAAAATCGCTTGGCGA
TTCTGGAGGGGATTGCACGAGGTGAACGTGCATTAGCTGACGATAAAGTGGTGAGTCACGATAAAGCCAAGGACAAAATG
TCAAAATGGCTGAAATAA