Detailed information of TA system TA220519

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 3776496..3777406
• Replicon: chromosome
• Accession: NZ_CP085143
• Organism: Halomonas sp. NyZ770

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LG409_RS17400
• Protein ID: WP_227407265.1
• Coordinates: 3776942..3777406 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LG409_RS17395
• Protein ID: WP_039178599.1
• Coordinates: 3776496..3776945 (+)
• Length: 150 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LG409_RS17370 (LG409_17370)	3771783..3772121	+	339	WP_096921856.1	hypothetical protein	-
LG409_RS17375 (LG409_17375)	3772314..3772709	+	396	WP_227407263.1	DUF2946 domain-containing protein	-
LG409_RS17380 (LG409_17380)	3772970..3774334	+	1365	WP_227407264.1	PepSY domain-containing protein	-
LG409_RS17385 (LG409_17385)	3774356..3774901	-	546	WP_081193388.1	copper chaperone PCu(A)C	-
LG409_RS17390 (LG409_17390)	3774920..3776284	-	1365	WP_039178596.1	diguanylate cyclase	-
LG409_RS17395 (LG409_17395)	3776496..3776945	+	450	WP_039178599.1	DUF2384 domain-containing protein	Antitoxin
LG409_RS17400 (LG409_17400)	3776942..3777406	+	465	WP_227407265.1	RES family NAD+ phosphorylase	Toxin
LG409_RS17405 (LG409_17405)	3777423..3778130	-	708	WP_227407266.1	urea ABC transporter ATP-binding subunit UrtE	-
LG409_RS17410 (LG409_17410)	3778130..3778966	-	837	WP_016915368.1	urea ABC transporter ATP-binding protein UrtD	-
LG409_RS17415 (LG409_17415)	3778963..3780105	-	1143	WP_016915369.1	urea ABC transporter permease subunit UrtC	-
LG409_RS17420 (LG409_17420)	3780112..3781719	-	1608	WP_227407267.1	urea ABC transporter permease subunit UrtB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (7-149)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (31-90)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 16897.23 Da
• Isoelectric Point: 4.4996

>T220519 WP_227407265.1 NZ_CP085143:3776942-3777406 [Halomonas sp. NyZ770]
MSEVTAFRLVKRKFQETAFDGDGARLYGGRWNSPGNACVYVASSESLALLEIMVHLESYRLLNAYALLRLTLPKQSILSV
GTEDLPENWREAPAPAETADLGDGWLASGQSLALALPSVVVPRELNYMLNPAHPLFEQAVASAEELPFQPDPRL

Nucleotide

• Length: 465 bp

>T220519 NZ_CP117053:4283556-4283813 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 150 a.a.
• Molecular weight: 16553.99 Da
• Isoelectric Point: 10.2591

>AT220519 WP_039178599.1 NZ_CP085143:3776496-3776945 [Halomonas sp. NyZ770]
MAATFSQYQPTKAVASGYWQQIGLPARGRKLHELLHEGVEYRVYPRLAKVSGIEQKALARYVDIPSATLSRRAKSGRFKM
AESDRLYRFAELFKAALDLFEGDAEGARAWLLKPNAGLGGRRPVEMSATSAEYQTVLNLIGRLEHGVSV

Nucleotide

• Length: 450 bp

>AT220519 NZ_CP117053:4283825-4284370 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA