Detailed information of TA system TA220518

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 3535081..3535691
• Replicon: chromosome
• Accession: NZ_CP085143
• Organism: Halomonas sp. NyZ770

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: LG409_RS16310
• Protein ID: WP_081138750.1
• Coordinates: 3535509..3535691 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LG409_RS16305
• Protein ID: WP_081138748.1
• Coordinates: 3535081..3535485 (-)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LG409_RS16295 (LG409_16295)	3531769..3534432	-	2664	WP_227407149.1	CRISPR-associated helicase Cas3'	-
LG409_RS16300 (LG409_16300)	3534419..3534790	-	372	WP_227408139.1	CRISPR-associated endonuclease Cas3''	-
LG409_RS16305 (LG409_16305)	3535081..3535485	-	405	WP_081138748.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LG409_RS16310 (LG409_16310)	3535509..3535691	-	183	WP_081138750.1	type II toxin-antitoxin system HicA family toxin	Toxin
LG409_RS16315 (LG409_16315)	3538402..3539835	-	1434	WP_227407150.1	PLP-dependent aminotransferase family protein	-
LG409_RS16320 (LG409_16320)	3540011..3540223	+	213	WP_081194578.1	DUF1127 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6749.89 Da
• Isoelectric Point: 10.7304

>T220518 WP_081138750.1 NZ_CP085143:c3535691-3535509 [Halomonas sp. NyZ770]
VKSKDVIKEMEADGWYLDRVSGSHHVFKHPTKLGSVPVPHPKKDLPKGTIHNIRKLAGLR

Nucleotide

• Length: 183 bp

>T220518 NZ_CP117053:4228829-4229170 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14725.58 Da
• Isoelectric Point: 4.6366

>AT220518 WP_081138748.1 NZ_CP085143:c3535485-3535081 [Halomonas sp. NyZ770]
MQYPVAIEWGDEHTATGIVIPDIPGAVTAGDTTEHAYEMAVEVAHIQLEELANENKDIPMPRSIAEHRQNPEFEGCGWGI
IDIDITPYLGKTEKVNVTLPGHVIRQIDQYVTTHGIKSRSAFIASAALEKLNHA

Nucleotide

• Length: 405 bp

>AT220518 NZ_CP117053:c4228834-4228758 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT