Detailed information of TA system TA220514

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-RHH
• Location: 2277814..2278346
• Replicon: chromosome
• Accession: NZ_CP085143
• Organism: Halomonas sp. NyZ770

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: LG409_RS10515
• Protein ID: WP_213262573.1
• Coordinates: 2278053..2278346 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A2M9TDT4
• Locus tag: LG409_RS10510
• Protein ID: WP_016914150.1
• Coordinates: 2277814..2278053 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LG409_RS10480 (LG409_10480)	2273039..2274013	-	975	WP_227406611.1	transcription termination factor Rho	-
LG409_RS10485 (LG409_10485)	2274082..2275419	-	1338	WP_227406612.1	PLP-dependent aminotransferase family protein	-
LG409_RS10490 (LG409_10490)	2275505..2276146	+	642	WP_227406613.1	DJ-1/PfpI family protein	-
LG409_RS10495 (LG409_10495)	2276198..2276788	-	591	WP_227406614.1	class I SAM-dependent methyltransferase	-
LG409_RS10500 (LG409_10500)	2276847..2277305	-	459	WP_227406615.1	hypothetical protein	-
LG409_RS10505 (LG409_10505)	2277507..2277719	-	213	WP_227408109.1	Txe/YoeB family addiction module toxin	-
LG409_RS10510 (LG409_10510)	2277814..2278053	+	240	WP_016914150.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
LG409_RS10515 (LG409_10515)	2278053..2278346	+	294	WP_213262573.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LG409_RS10520 (LG409_10520)	2278425..2279339	-	915	WP_227406616.1	SH3 domain-containing protein	-
LG409_RS10525 (LG409_10525)	2279397..2280290	-	894	WP_227406617.1	hypothetical protein	-
LG409_RS10530 (LG409_10530)	2280356..2280886	-	531	WP_227406618.1	hypothetical protein	-
LG409_RS10535 (LG409_10535)	2281019..2281735	-	717	WP_227406619.1	SDR family oxidoreductase	-
LG409_RS10540 (LG409_10540)	2282515..2283126	-	612	WP_227406620.1	cob(I)yrinic acid a,c-diamide adenosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

• Antitoxin ParD (1-77)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11319.97 Da
• Isoelectric Point: 10.6179

>T220514 WP_213262573.1 NZ_CP085143:2278053-2278346 [Halomonas sp. NyZ770]
MRPYLLTASARKDVVEIGRFTTEKWGKRQRDTYLRQLDDAFKRLARQPDIGRDAGDIKPGYKKFSQGSHVIFYRAGTESR
IVVIRILHNSMDVEQHL

Nucleotide

• Length: 294 bp

>T220514 NZ_CP117053:3571419-3571637 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8863.83 Da
• Isoelectric Point: 4.3041

>AT220514 WP_016914150.1 NZ_CP085143:2277814-2278053 [Halomonas sp. NyZ770]
MARNTSITLGPHFDEFIATQVEKGRYGSVSEVVRAGLRLLEETESKLERLQRLLDEGEQSGIAEYSLESVITELDNEAH

Nucleotide

• Length: 240 bp

>AT220514 NZ_CP117053:3571019-3571393 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A2M9TDT4