Detailed information of TA system TA220429

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 563915..564589
• Replicon: chromosome
• Accession: NZ_CP085087
• Organism: Streptococcus suis strain Ssuis_MA2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: V6Z2K9
• Locus tag: LIO32_RS02935
• Protein ID: WP_015647112.1
• Coordinates: 563915..564097 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LIO32_RS02940
• Protein ID: WP_074389768.1
• Coordinates: 564137..564589 (+)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIO32_RS02895	559034..559786	+	753	WP_044758031.1	DnaD domain protein	-
LIO32_RS02900	559796..560623	+	828	WP_024381267.1	ATP-binding protein	-
LIO32_RS02905	561065..561247	+	183	WP_024405996.1	hypothetical protein	-
LIO32_RS02910	561339..561950	+	612	WP_024405995.1	hypothetical protein	-
LIO32_RS02915	561997..562425	+	429	WP_074389766.1	replication protein	-
LIO32_RS02920	562433..562744	+	312	WP_074389767.1	hypothetical protein	-
LIO32_RS02925	562762..563175	+	414	WP_024405993.1	hypothetical protein	-
LIO32_RS02935	563915..564097	+	183	WP_015647112.1	type II toxin-antitoxin system HicA family toxin	Toxin
LIO32_RS02940	564137..564589	+	453	WP_074389768.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LIO32_RS02945	564703..564912	+	210	WP_015647110.1	hypothetical protein	-
LIO32_RS02950	565756..565977	+	222	WP_012775256.1	DUF6290 family protein	-
LIO32_RS02955	565979..566239	+	261	WP_012027435.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LIO32_RS02960	566406..567047	+	642	WP_023370899.1	HAD family hydrolase	-
LIO32_RS02965	567080..567265	-	186	WP_002935693.1	hypothetical protein	-
LIO32_RS02970	567329..568333	-	1005	WP_023370897.1	lactonase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	555406..570854	15448

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (18-80)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6630.84 Da
• Isoelectric Point: 10.8207

>T220429 WP_015647112.1 NZ_CP085087:563915-564097 [Streptococcus suis]
MPMTQKEMVKLLTANGWTKTKGGKGSHVKLEKAGERPITIPHGEINKYTERGIKKQAGLL

Nucleotide

• Length: 183 bp

>T220429 NZ_CP117020:2440791-2441255 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTACAGCGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAGCCCATTGA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16723.73 Da
• Isoelectric Point: 3.9495

>AT220429 WP_074389768.1 NZ_CP085087:564137-564589 [Streptococcus suis]
MLVTYPALFYYDDTDGASAPYFVTFPDFEHSATQGEDMANAMAMASDWLGIHLADYIENGRDIPIPTPINTLSLADNNPF
HDDEDIELIYDPSKSFISMVMVDVAEYLGSQEPIKKTLTIPRWADTLGRELGLNFSQTLTDAIADKKIHA

Nucleotide

• Length: 453 bp

>AT220429 NZ_CP117020:2440456-2440791 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACACTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V6Z2K9