Detailed information of TA system TA220419

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-couple_hipB
• Location: 1025454..1026697
• Replicon: chromosome
• Accession: NZ_CP085085
• Organism: Streptococcus suis strain Ssuis_MA8

Toxin (Protein)

• Gene name: sezT
• Uniprot ID: G7SEB4
• Locus tag: LIO34_RS05235
• Protein ID: WP_001235242.1
• Coordinates: 1025454..1026221 (-)
• Length: 256 a.a.

Antitoxin (Protein)

• Gene name: sezA
• Uniprot ID: -
• Locus tag: LIO34_RS05240
• Protein ID: WP_000578719.1
• Coordinates: 1026221..1026697 (-)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIO34_RS05210	1020829..1023297	-	2469	Protein_981	topoisomerase DNA-binding C4 zinc finger domain-containing protein	-
LIO34_RS05215	1023467..1024204	-	738	WP_001038796.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
LIO34_RS05220	1024329..1024409	-	81	WP_032501554.1	23S rRNA methyltransferase attenuator leader peptide ErmL	-
LIO34_RS05225	1024475..1024708	-	234	WP_020220349.1	peptide-binding protein	-
LIO34_RS05230	1024824..1025375	-	552	Protein_985	topoisomerase DNA-binding C4 zinc finger domain-containing protein	-
LIO34_RS05235	1025454..1026221	-	768	WP_001235242.1	type II toxin-antitoxin system toxin PezT	Toxin
LIO34_RS05240	1026221..1026697	-	477	WP_000578719.1	type II toxin-antitoxin system antitoxin PezA	Antitoxin
LIO34_RS05245	1026767..1027057	-	291	WP_000667951.1	hypothetical protein	-
LIO34_RS05250	1027108..1027497	-	390	WP_000048066.1	DUF5945 family protein	-
LIO34_RS05255	1027494..1027721	-	228	WP_000110922.1	DUF5965 family protein	-
LIO34_RS05260	1027749..1028849	-	1101	WP_000196860.1	toprim domain-containing protein	-
LIO34_RS05265	1028913..1030769	-	1857	WP_001820670.1	recombinase family protein	-
LIO34_RS05270	1030824..1031087	-	264	WP_000253176.1	hypothetical protein	-
LIO34_RS05275	1031087..1031317	-	231	WP_001206986.1	PC4/YdbC family ssDNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	erm(B) / tet(O)	-	1004806..1039858	35052

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (28-214)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 256 a.a.
• Molecular weight: 28985.80 Da
• Isoelectric Point: 5.1126

>T220419 WP_001235242.1 NZ_CP085085:c1026221-1025454 [Streptococcus suis]
MRLEEFSEAEFQKALQRTIRALTRGKTIPDQPKAILLGGQSGAGKTTIHRIKQKEFQGNIIIIDGDSYRSQHPNYLALQE
KYGKDSVDYTKGFAGKMVEQLVDELSTQGYHLLIEGTLRTTQVPRQTAQLLSSKGYQVSLAVIGTKPELSYLSTLIRYEE
LYAIDPTQARATPKEHHDGIVENLVDNLRELESENLFDQIQIYQRDRTCIYDSETAEGSAAEVLQECLFGKWSKVEEEMV
KESELLLKELLEKNK

Nucleotide

• Length: 768 bp

>T220419 NZ_CP117020:c338659-338552 [Escherichia coli]
ATGACGCTCACGCAGTTTGCCATGACTTTCTGGCACGACTTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18266.77 Da
• Isoelectric Point: 4.6424

>AT220419 WP_000578719.1 NZ_CP085085:c1026697-1026221 [Streptococcus suis]
MIGDNIKSLRRTHDLTQPEFAKMVGISRNSLSRYENGTSTVSTELIDRICQKFNVSYVDIVGEDKMLTPVEDYHLTLKVE
VIKERGAAILSKLYRYQDSQDIVFDDESNPWILMSDDLAELINTKIYLVATFDEIERYNGYLDGIERMLDMVHHRVVA

Nucleotide

• Length: 477 bp

>AT220419 NZ_CP117020:338707-338773 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTATACCTCTCAACGTGCGGGGGTTTTCTC

Structures

Toxin

• AlphaFold DB: G7SEB4