220414

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/HTH_IclR(antitoxin)
Location 3924355..3926050 Replicon chromosome
Accession NZ_CP085082
Organism Pseudomonas aeruginosa strain PAO1-Holloway

Toxin (Protein)


Gene name doc Uniprot ID Q9HYA0
Locus tag LIO20_RS18215 Protein ID WP_003112901.1
Coordinates 3925181..3926050 (-) Length 290 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID Q9HYA1
Locus tag LIO20_RS18210 Protein ID WP_003112902.1
Coordinates 3924355..3925188 (-) Length 278 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LIO20_RS18190 (LIO20_18150) 3919586..3921070 - 1485 WP_003112906.1 aldehyde dehydrogenase -
LIO20_RS18195 (LIO20_18155) 3921082..3921885 - 804 WP_003112905.1 aspartate dehydrogenase -
LIO20_RS18200 (LIO20_18160) 3921896..3923578 - 1683 WP_003112904.1 thiamine pyrophosphate-binding protein -
LIO20_RS18205 (LIO20_18165) 3923571..3924368 - 798 WP_003112903.1 SDR family oxidoreductase -
LIO20_RS18210 (LIO20_18170) 3924355..3925188 - 834 WP_003112902.1 IclR family transcriptional regulator Antitoxin
LIO20_RS18215 (LIO20_18175) 3925181..3926050 - 870 WP_003112901.1 alpha/beta hydrolase Toxin
LIO20_RS18220 (LIO20_18180) 3926059..3926589 - 531 WP_003112900.1 cupin domain-containing protein -
LIO20_RS18225 (LIO20_18185) 3926603..3927364 - 762 WP_003112899.1 SDR family oxidoreductase -
LIO20_RS18230 (LIO20_18190) 3927370..3928146 - 777 WP_003112898.1 ABC transporter permease -
LIO20_RS18235 (LIO20_18195) 3928143..3929150 - 1008 WP_003112897.1 ABC transporter substrate-binding protein -
LIO20_RS18240 (LIO20_18200) 3929191..3930042 - 852 WP_003130104.1 ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Alpha/beta hydrolase fold-1

(41-272)

Antitoxin

Transcription regulator IclR, N-terminal

(16-66)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 30403.60 Da        Isoelectric Point: 6.5894

>T220414 WP_003112901.1 NZ_CP085082:c3926050-3925181 [Pseudomonas aeruginosa]
MNMAQTHTPVPNLQQRFPERLVQLADGAQLAIRECGQGPVVVLLHGIGSGSASWLHCAQRLAAGNRVIAWDAPGYGLSTP
LPPARPKACDYAACLELLLDALGVESCLLVGHSLGALMATAYAAGIGAARVRRLVLLSPARGYGAAELRDSGAQVRRQRL
ENLERFGIDGMASERTARLLGRNPSEEALAWVRWNMARLNPEGYRQAVELLCGDDLLGNGQPAAPCEVHCGEDDGITTPE
SCGAIARQLGASFSSIPGAGHASPIEQPEVVAGRIGHAQRLSLEGTANG

Download         Length: 870 bp

>T220414 NZ_CP117016:c4999670-4999014 [Escherichia coli]
ATGGCTTATGATTACAGCGCCAGCCTCAACCCGCAAAAAGCTCTGATCTGGCGTATCGTCCATCGGGACAATATCCCGTG
GATTCTGGATAACGGGCTGCACTGCGGCAACAGTCTGGTGCAGGCGGAAAACTGGATCAATATCGGCAACCCGGAGCTGA
TCGGCAAGCGTGCCGGGCATCCGGTACCTGTCGGAACGGGCGGGACACTGCATGACTATGTGCCGTTTTATTTTACCCCC
TTCTCGCCAATGCTGATGAACATCCACAGCGGGCGCGGCGGCATTAAGCAACGCCCCAATGAGGAGATCGTTATTCTGGT
CAGCAACTTGCGAAACGTGGCAGCACACGACGTACCCTTCGTGTTTACCGACAGCCATGCTTACTATAACTGGACCAACT
ACTACACCAGTCTGGATAGTCTGGACCAGATCGACTGGCCCATTTTGCAGGCACGGGACTTTCGCCGCGATCCAGACGAT
CCGGCTAAATTTGAACGCTATCAGGCAGAGGCGCTAATCTGGCAACACTGCCCGATCTCTCTGCTGGATGGGATAATTTG
TTACAGCGAAGAGGTCAGGTTACAATTAGAACAATGGCTGTTTCAGCGTAATCTAACAATGTCCGTTCACACACGGGCTG
GATGGTACTTCTCATGA

Antitoxin


Download         Length: 278 a.a.        Molecular weight: 30877.26 Da        Isoelectric Point: 5.7725

>AT220414 WP_003112902.1 NZ_CP085082:c3925188-3924355 [Pseudomonas aeruginosa]
MDKSDDSQDKYIVPGLERGLLLLCEFSRKDRTLTAPELARRLKLPRSTIFRLLTTLEAMGFVTRNGNEYRLGMAVLRLGF
EYLASLELTELGQPLLNRLCDEIRYPCNLVVRDGRSIVYVAKVSPSTPLSSSVNVGTRLPAHATVLGRILLQDLSLGELR
ELYPEEQLEQFSPNTPRSVLELFDMVQGDRQRGFVQGEGFFEASISTVAAPVRDHSGRVIAAMGATIAAGHIDPERIEGL
VSRVRSSADELSYLLDYRADGQDNVTPIFRSRPHETV

Download         Length: 834 bp

>AT220414 NZ_CP117016:c4999017-4997950 [Escherichia coli]
ATGATCACATATACACAAGGCAATTTACTGGACGCACCGGTGGAAGCTCTGGTGAATACGGTTAATACCGTCGGTGTCAT
GGGGAAAGGCATCGCGTTGATGTTTAAAGAGCGCTTCCCCGAAAATATGAAAGTCTATGCCCTCGCCTGCAAACAAAAGC
AGGTGATCACCGGCAAAATGTTCATTACCGAAACCGGGGAACTGATGGGACCGCGCTGGATTGTTAATTTTCCGACCAAA
CAGCACTGGCGCGCTGATTCCCGCATGGAGTGGATTGAAGATGGCTTGCAGGACTTACGCCGCTTTTTGATTGAAGAGAA
CGTGCAGTCCATCGCCATTCCACCACTGGGCGCAGGCAACGGTGGTTTGAACTGGCCGGACGTTCGGGCGCAGATTGAAT
CTGCCCTTGGCGACCTTCAGGACGTTGATATCCTGATTTACCAACCGACTGAAAAATACCAGAACGTTGCCAAAAGCACT
GGCGTAAAAAAGCTCACTCCCGCCAGAGCCGCCATCGCTGAACTGGTGCGTCGCTACTGGGTATTGGGGATGGAGTGCAG
CCTACTGGAAATCCAGAAACTGGCCTGGCTGTTACAACGCGCCATTGAGCAGCATCAACAGGACGATATCCTCAAACTGC
GGTTTGAAGCTCACTATTATGGCCCCTATGCGCCCAATCTGAACCATCTGCTTAATGCGCTGGACGGCACCTATCTTAAA
GCCGAGAAACGCATCCCGGACAGCCAACCGCTGGATGTGATCTGGTTTAACGATCAGAAAAAAGAACACGTTAATGCGTA
TCTGAACAACGAAGCCAGAGAGTGGCTACCTGCGCTGGAGCAGGTCAGCCAGTTAATTGACGGCTTTGAATCGCCGTTTG
GTCTGGAGTTGCTGGCAACCGTAGACTGGTTGCTGAGCCGTGGCGAATGTCAACCAACGCTGGATTCCGTTAAGGAAGGG
CTGCACCAGTGGCCTGCGGGCGAACGCTGGGCCAGCCGTAAGTTGAGGCTGTTTGATAATAATAATCTGCAATTTGCCAT
TAACCGCGTAATGGAGTTCCATTGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q9HYA0

Antitoxin

Source ID Structure
AlphaFold DB Q9HYA1

References