220413

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /Acetyltransf_4(toxin)
Location 3658060..3659568 Replicon chromosome
Accession NZ_CP085082
Organism Pseudomonas aeruginosa strain PAO1-Holloway

Toxin (Protein)


Gene name - Uniprot ID Q9HYX1
Locus tag LIO20_RS16975 Protein ID WP_003119682.1
Coordinates 3658981..3659568 (-) Length 196 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q9HYX2
Locus tag LIO20_RS16970 Protein ID WP_003115566.1
Coordinates 3658060..3658914 (-) Length 285 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LIO20_RS16955 (LIO20_16920) 3653479..3653688 + 210 WP_003115568.1 cold-shock protein -
LIO20_RS16960 (LIO20_16925) 3653749..3655623 - 1875 WP_003091605.1 MFS transporter -
LIO20_RS16965 (LIO20_16930) 3655798..3657963 - 2166 WP_003115567.1 TonB-dependent receptor family protein -
LIO20_RS16970 (LIO20_16935) 3658060..3658914 - 855 WP_003115566.1 AraC family transcriptional regulator Antitoxin
LIO20_RS16975 (LIO20_16940) 3658981..3659568 - 588 WP_003119682.1 GNAT family protein Toxin
LIO20_RS16980 (LIO20_16945) 3659834..3663313 + 3480 WP_003114080.1 PAS domain-containing hybrid sensor histidine kinase/response regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(20-152)

Antitoxin

AraC-type arabinose-binding/dimerisation domain

(19-151)


Sequences

Toxin        


Download         Length: 196 a.a.        Molecular weight: 21901.23 Da        Isoelectric Point: 9.8676

>T220413 WP_003119682.1 NZ_CP085082:c3659568-3658981 [Pseudomonas aeruginosa]
MSRRAPMFKPLPITLQRGALRLEPLVEADIPELVSLAEANREALQYMDGPTRPDWYRQSLAEQREGRALPLAVRLGVQLV
GTTRFAEFLPALPACEIGWTWLDQAQHGSGLNRMIKYLMLKHAFDNLRMVRVQLSTAASNLRAQGAIDKLGAQREGVLRN
HRRLAGGRLDDTFVYSITDHEWPQVKAALEASFTG

Download         Length: 588 bp

>T220413 NZ_CP117016:c4302909-4302691 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 285 a.a.        Molecular weight: 31933.23 Da        Isoelectric Point: 7.3276

>AT220413 WP_003115566.1 NZ_CP085082:c3658914-3658060 [Pseudomonas aeruginosa]
VPDETSGEHTHFWRAAELGGVELLHARYVEQRFAPHVHSSFALVIIEQGAQRFRHRGGEHLAPLGSMVLINPDEVHTGSK
AHDAGWLYRGYYPEMEQIDGILDELEIHHGGVPRFADSVIHDQPLARAFVHLHRLLAEHADSLETQTRWRETVLALFRRH
ARLPEAPKAGQEPLAVARARELLAARLLDPPSLEELAAAVNLSPFHFARVFRRATGMPPHAWLRQRRLEQARALLKSGRA
PLEVALELGFADQSHLGRQFKQAYGVAPGEYRSACARSSLTIYE

Download         Length: 855 bp

>AT220413 NZ_CP117016:c4303309-4302935 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1YRE
PDB 8GXJ

Antitoxin

Source ID Structure
AlphaFold DB Q9HYX2

References