Detailed information of TA system TA220412

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 2048360..2049581
• Replicon: chromosome
• Accession: NZ_CP085082
• Organism: Pseudomonas aeruginosa strain PAO1-Holloway

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LIO20_RS09715
• Protein ID: WP_003114949.1
• Coordinates: 2048360..2048938 (-)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LIO20_RS09720
• Protein ID: WP_003088112.1
• Coordinates: 2049027..2049581 (-)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIO20_RS09700 (LIO20_09695)	2043637..2044914	+	1278	WP_003102743.1	TolC family protein	-
LIO20_RS09705 (LIO20_09700)	2044904..2047075	+	2172	WP_003114950.1	type I secretion system permease/ATPase	-
LIO20_RS09710 (LIO20_09705)	2047065..2048252	+	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
LIO20_RS09715 (LIO20_09710)	2048360..2048938	-	579	WP_003114949.1	HD domain-containing protein	Toxin
LIO20_RS09720 (LIO20_09715)	2049027..2049581	-	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
LIO20_RS09725 (LIO20_09720)	2049739..2051934	-	2196	WP_003114948.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
LIO20_RS09730 (LIO20_09725)	2051939..2052400	-	462	WP_003102736.1	(2Fe-2S)-binding protein	-
LIO20_RS09735 (LIO20_09730)	2052731..2053054	+	324	WP_003102734.1	multidrug efflux SMR transporter	-
LIO20_RS09740 (LIO20_09735)	2053067..2053465	+	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
LIO20_RS09745 (LIO20_09740)	2053462..2054013	-	552	WP_003102727.1	XRE family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21897.75 Da
• Isoelectric Point: 5.3655

>T220412 WP_003114949.1 NZ_CP085082:c2048938-2048360 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAVRRDA

Nucleotide

• Length: 579 bp

>T220412 NZ_CP117016:4080286-4080684 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTTGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT220412 WP_003088112.1 NZ_CP085082:c2049581-2049027 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT220412 NZ_CP117016:4079990-4080283 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA