Detailed information of TA system TA220411

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 1115917..1117175
• Replicon: chromosome
• Accession: NZ_CP085082
• Organism: Pseudomonas aeruginosa strain PAO1-Holloway

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9I4U4
• Locus tag: LIO20_RS05335
• Protein ID: WP_003112528.1
• Coordinates: 1116420..1117175 (+)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: LIO20_RS05330
• Protein ID: WP_003086329.1
• Coordinates: 1115917..1116285 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIO20_RS05310 (LIO20_05310)	1112080..1112355	+	276	WP_010793902.1	zinc ribbon domain-containing protein	-
LIO20_RS05315 (LIO20_05315)	1112358..1112834	-	477	WP_003112531.1	hypothetical protein	-
LIO20_RS05320 (LIO20_05320)	1112913..1114502	+	1590	WP_010895535.1	aldehyde dehydrogenase family protein	-
LIO20_RS05325 (LIO20_05325)	1114558..1115844	+	1287	WP_003112529.1	FAD-binding oxidoreductase	-
LIO20_RS05330 (LIO20_05330)	1115917..1116285	+	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
LIO20_RS05335 (LIO20_05335)	1116420..1117175	+	756	WP_003112528.1	RES family NAD+ phosphorylase	Toxin
LIO20_RS05340 (LIO20_05340)	1117943..1119304	+	1362	WP_010895536.1	DNA recombination protein RmuC	-
LIO20_RS05345 (LIO20_05345)	1119459..1122002	-	2544	WP_003112526.1	acyl-homoserine-lactone acylase QuiP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28278.42 Da
• Isoelectric Point: 7.7294

>T220411 WP_003112528.1 NZ_CP085082:1116420-1117175 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T220411 NZ_CP117016:c3798534-3798382 [Escherichia coli]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT220411 WP_003086329.1 NZ_CP085082:1115917-1116285 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT220411 NZ_CP117016:3798583-3798640 [Escherichia coli]
GTTCAGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Structures

Toxin

• AlphaFold DB: Q9I4U4

Antitoxin

• AlphaFold DB: V6AJU7