Detailed information of TA system TA220407

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 4095605..4096386
• Replicon: chromosome
• Accession: NZ_CP085080
• Organism: Enterobacter cloacae strain ECl_1.VN

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: LH408_RS19325
• Protein ID: WP_226835104.1
• Coordinates: 4095605..4096096 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A5E1AUV7
• Locus tag: LH408_RS19330
• Protein ID: WP_029882966.1
• Coordinates: 4096093..4096386 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LH408_RS19305 (LH408_19305)	4091355..4092047	+	693	WP_038983516.1	response regulator	-
LH408_RS19310 (LH408_19310)	4092136..4094271	-	2136	WP_226835102.1	ornithine decarboxylase	-
LH408_RS19315 (LH408_19315)	4094454..4095167	+	714	WP_013098689.1	DUF554 domain-containing protein	-
LH408_RS19325 (LH408_19325)	4095605..4096096	-	492	WP_226835104.1	GNAT family N-acetyltransferase	Toxin
LH408_RS19330 (LH408_19330)	4096093..4096386	-	294	WP_029882966.1	DUF1778 domain-containing protein	Antitoxin
LH408_RS19335 (LH408_19335)	4096635..4098422	-	1788	WP_062729673.1	hybrid sensor histidine kinase/response regulator	-
LH408_RS19340 (LH408_19340)	4098419..4099051	-	633	WP_013098693.1	response regulator transcription factor	-
LH408_RS19345 (LH408_19345)	4099227..4099541	+	315	WP_013098694.1	lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17656.55 Da
• Isoelectric Point: 7.2653

>T220407 WP_226835104.1 NZ_CP085080:c4096096-4095605 [Enterobacter cloacae]
MISAPEPLHAGHILTPFCCGVDSMDNWLKQRAMKNQVSGASRTFVCCGNDSNVLAYYSLASSAVMTNTAPGRFRRNMPDP
IPVVVLGRLAVDQSLHGQGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEAREFYLRVGFEPSPMDPMILMVTLGDLM
ANI

Nucleotide

• Length: 492 bp

>T220407 NZ_CP117016:3408762-3408935 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTAGGAATAACCTGGCCAATGAGTTTACCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11003.66 Da
• Isoelectric Point: 9.9156

>AT220407 WP_029882966.1 NZ_CP085080:c4096386-4096093 [Enterobacter cloacae]
MPAANTMAMKRETLNLRIRPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPEAYQEFLARLDQAPAPN
ATLRKTMQTPAPWEREK

Nucleotide

• Length: 294 bp

>AT220407 NZ_CP117016:3408438-3408734 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGCATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A5E1AUV7