Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 645649..646225 Replicon chromosome
Accession NZ_CP085080
Organism Enterobacter cloacae strain ECl_1.VN

Toxin (Protein)


Gene name shpA Uniprot ID A0A5E1APA0
Locus tag LH408_RS03095 Protein ID WP_023620650.1
Coordinates 645649..645936 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A5E1AP18
Locus tag LH408_RS03100 Protein ID WP_023620649.1
Coordinates 645923..646225 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LH408_RS03075 (LH408_03075) 641611..642081 + 471 WP_013095432.1 MarR family transcriptional regulator -
LH408_RS03080 (LH408_03080) 642078..643145 + 1068 WP_029882194.1 HlyD family secretion protein -
LH408_RS03085 (LH408_03085) 643135..644211 + 1077 WP_226835639.1 DUF2955 domain-containing protein -
LH408_RS03090 (LH408_03090) 644181..645479 - 1299 WP_062856502.1 DUF445 domain-containing protein -
LH408_RS03095 (LH408_03095) 645649..645936 + 288 WP_023620650.1 BrnT family toxin Toxin
LH408_RS03100 (LH408_03100) 645923..646225 + 303 WP_023620649.1 BrnA antitoxin family protein Antitoxin
LH408_RS03105 (LH408_03105) 646256..646894 - 639 WP_226835641.1 LysE family translocator -
LH408_RS03110 (LH408_03110) 646943..647686 - 744 WP_226835643.1 AraC family transcriptional regulator -
LH408_RS03115 (LH408_03115) 647823..649193 + 1371 WP_226835645.1 NAD-dependent succinate-semialdehyde dehydrogenase -
LH408_RS03120 (LH408_03120) 649235..649555 - 321 WP_226835646.1 hypothetical protein -
LH408_RS03125 (LH408_03125) 649555..650100 - 546 WP_013095440.1 YfaZ family outer membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11246.60 Da        Isoelectric Point: 6.8813

>T220394 WP_023620650.1 NZ_CP085080:645649-645936 [Enterobacter cloacae]
MPTEYEWDSDKAKSNQQKHGIRFEDAVLVFDDPYHLSVQDRYENGEFRWQTIGLVQGVIVILVAHTVRFESGGEIIRIIS
ARKADRKERNRYEHR

Download         Length: 288 bp

>T220394 NZ_CP117013:c4591828-4591454 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11414.05 Da        Isoelectric Point: 10.3694

>AT220394 WP_023620649.1 NZ_CP085080:645923-646225 [Enterobacter cloacae]
MSIVKHKRGSSPEVSARRDAELKALAEKPEAEIDYSDIPATDDKQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT220394 NZ_CP117013:c4592286-4591918 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1APA0

Antitoxin

Source ID Structure
AlphaFold DB A0A5E1AP18

References