Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 121064..121689 Replicon plasmid pNDM_P21_SE1_04.20
Accession NZ_CP085075
Organism Escherichia coli strain P21_SE1_04.20

Toxin (Protein)


Gene name vapC Uniprot ID A0A1D7QA53
Locus tag LH372_RS24765 Protein ID WP_039023147.1
Coordinates 121064..121462 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1D7QA08
Locus tag LH372_RS24770 Protein ID WP_039023146.1
Coordinates 121462..121689 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LH372_RS24750 (117371) 117371..117868 + 498 WP_000605862.1 entry exclusion protein -
LH372_RS24755 (117900) 117900..118631 + 732 WP_000850422.1 conjugal transfer complement resistance protein TraT -
LH372_RS24760 (118884) 118884..121055 + 2172 WP_121350052.1 type IV conjugative transfer system coupling protein TraD -
LH372_RS24765 (121064) 121064..121462 - 399 WP_039023147.1 type II toxin-antitoxin system VapC family toxin Toxin
LH372_RS24770 (121462) 121462..121689 - 228 WP_039023146.1 toxin-antitoxin system antitoxin VapB Antitoxin

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / rmtB / blaTEM-1B / blaCTX-M-15 / mph(A) / aadA5 - 1..134984 134984


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-43)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14885.26 Da        Isoelectric Point: 8.2824

>T220385 WP_039023147.1 NZ_CP085075:c121462-121064 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAAIHT
GQIRAELARQGRPVGPFDQMIAGHARCRGLIIVTNNTREFERVGGLRIEDWS

Download         Length: 399 bp

>T220385 NZ_CP117013:2304598-2304701 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8549.62 Da        Isoelectric Point: 4.7513

>AT220385 WP_039023146.1 NZ_CP085075:c121689-121462 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAIGRTRLITPAGETWDEWFDGHSVSADFMDNREQPGMQERESF

Download         Length: 228 bp

>AT220385 NZ_CP117013:c2304739-2304471 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCTG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D7QA53

Antitoxin

Source ID Structure
AlphaFold DB A0A1D7QA08

References