Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 61775..62300 Replicon plasmid pNDM_P21_SE1_04.20
Accession NZ_CP085075
Organism Escherichia coli strain P21_SE1_04.20

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag LH372_RS24405 Protein ID WP_001159871.1
Coordinates 61995..62300 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag LH372_RS24400 Protein ID WP_000813630.1
Coordinates 61775..61993 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LH372_RS24375 (56985) 56985..57671 + 687 Protein_66 IS1 family transposase -
LH372_RS24380 (57925) 57925..58947 - 1023 WP_000361402.1 helicase UvrD -
LH372_RS24385 (58932) 58932..60497 - 1566 WP_001128474.1 AAA family ATPase -
LH372_RS24390 (60572) 60572..60988 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
LH372_RS24395 (60985) 60985..61215 - 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
LH372_RS24400 (61775) 61775..61993 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
LH372_RS24405 (61995) 61995..62300 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
LH372_RS24410 (62301) 62301..63107 + 807 WP_226907401.1 site-specific integrase -
LH372_RS24415 (63829) 63829..64584 + 756 WP_000852146.1 replication initiation protein RepE -
LH372_RS24420 (65172) 65172..66338 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / rmtB / blaTEM-1B / blaCTX-M-15 / mph(A) / aadA5 - 1..134984 134984


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T220381 WP_001159871.1 NZ_CP085075:61995-62300 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T220381 NZ_CP117013:1071208-1071615 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCTTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT220381 WP_000813630.1 NZ_CP085075:61775-61993 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT220381 NZ_CP117013:1070961-1071227 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References