Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 60572..61215 Replicon plasmid pNDM_P21_SE1_04.20
Accession NZ_CP085075
Organism Escherichia coli strain P21_SE1_04.20

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag LH372_RS24390 Protein ID WP_001034044.1
Coordinates 60572..60988 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag LH372_RS24395 Protein ID WP_001261286.1
Coordinates 60985..61215 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LH372_RS24365 (55825) 55825..55938 - 114 Protein_64 IS6 family transposase -
LH372_RS24370 (56138) 56138..56835 + 698 WP_223350503.1 IS1 family transposase -
LH372_RS24375 (56985) 56985..57671 + 687 Protein_66 IS1 family transposase -
LH372_RS24380 (57925) 57925..58947 - 1023 WP_000361402.1 helicase UvrD -
LH372_RS24385 (58932) 58932..60497 - 1566 WP_001128474.1 AAA family ATPase -
LH372_RS24390 (60572) 60572..60988 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
LH372_RS24395 (60985) 60985..61215 - 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LH372_RS24400 (61775) 61775..61993 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
LH372_RS24405 (61995) 61995..62300 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
LH372_RS24410 (62301) 62301..63107 + 807 WP_226907401.1 site-specific integrase -
LH372_RS24415 (63829) 63829..64584 + 756 WP_000852146.1 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / rmtB / blaTEM-1B / blaCTX-M-15 / mph(A) / aadA5 - 1..134984 134984


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T220380 WP_001034044.1 NZ_CP085075:c60988-60572 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T220380 NZ_CP085075:c60988-60572 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT220380 WP_001261286.1 NZ_CP085075:c61215-60985 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT220380 NZ_CP085075:c61215-60985 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References