Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4340780..4341375 Replicon chromosome
Accession NZ_CP085074
Organism Escherichia coli strain P21_SE1_04.20

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag LH372_RS21050 Protein ID WP_000239579.1
Coordinates 4340780..4341130 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag LH372_RS21055 Protein ID WP_001223208.1
Coordinates 4341124..4341375 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LH372_RS21030 (4336226) 4336226..4337248 - 1023 WP_001295197.1 ABC transporter permease -
LH372_RS21035 (4337262) 4337262..4338764 - 1503 WP_000205784.1 sugar ABC transporter ATP-binding protein -
LH372_RS21040 (4338904) 4338904..4339860 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
LH372_RS21045 (4340170) 4340170..4340700 + 531 WP_000055072.1 inorganic diphosphatase -
LH372_RS21050 (4340780) 4340780..4341130 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
LH372_RS21055 (4341124) 4341124..4341375 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
LH372_RS21060 (4341587) 4341587..4341928 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
LH372_RS21065 (4341931) 4341931..4345710 - 3780 WP_000060978.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T220378 WP_000239579.1 NZ_CP085074:c4341130-4340780 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T220378 NZ_CP117011:35375-35527 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT220378 WP_001223208.1 NZ_CP085074:c4341375-4341124 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT220378 NZ_CP117011:c35323-35263 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References