Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4225520..4226096 Replicon chromosome
Accession NZ_CP085074
Organism Escherichia coli strain P21_SE1_04.20

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag LH372_RS20455 Protein ID WP_001297643.1
Coordinates 4225809..4226096 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag LH372_RS20450 Protein ID WP_000063160.1
Coordinates 4225520..4225822 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LH372_RS20435 (4222116) 4222116..4224266 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
LH372_RS20440 (4224316) 4224316..4224519 + 204 WP_000467859.1 YbdD/YjiX family protein -
LH372_RS20445 (4224530) 4224530..4225486 + 957 WP_001297640.1 GTPase -
LH372_RS20450 (4225520) 4225520..4225822 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
LH372_RS20455 (4225809) 4225809..4226096 - 288 WP_001297643.1 BrnT family toxin Toxin
LH372_RS20460 (4226295) 4226295..4226991 - 697 Protein_4004 IS1 family transposase -
LH372_RS20465 (4227306) 4227306..4228538 + 1233 WP_001137030.1 multidrug efflux MFS transporter MdtM -
LH372_RS20470 (4228579) 4228579..4229859 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - cheD 4132165..4226504 94339
- flank IS/Tn - - 4226295..4226504 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T220377 WP_001297643.1 NZ_CP085074:c4226096-4225809 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T220377 NZ_CP117010:c4174362-4174189 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT220377 WP_000063160.1 NZ_CP085074:c4225822-4225520 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT220377 NZ_CP117010:c4174686-4174390 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References