Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3631501..3632119 Replicon chromosome
Accession NZ_CP085074
Organism Escherichia coli strain P21_SE1_04.20

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LH372_RS17580 Protein ID WP_001291435.1
Coordinates 3631901..3632119 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LH372_RS17575 Protein ID WP_000344800.1
Coordinates 3631501..3631875 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LH372_RS17565 (3626590) 3626590..3627783 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LH372_RS17570 (3627806) 3627806..3630955 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
LH372_RS17575 (3631501) 3631501..3631875 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LH372_RS17580 (3631901) 3631901..3632119 + 219 WP_001291435.1 HHA domain-containing protein Toxin
LH372_RS17585 (3632291) 3632291..3632842 + 552 WP_000102564.1 maltose O-acetyltransferase -
LH372_RS17590 (3632958) 3632958..3633428 + 471 WP_000136192.1 YlaC family protein -
LH372_RS17595 (3633592) 3633592..3635142 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LH372_RS17600 (3635184) 3635184..3635537 - 354 WP_000878140.1 DUF1428 domain-containing protein -
LH372_RS17610 (3635916) 3635916..3636227 + 312 WP_000409911.1 MGMT family protein -
LH372_RS17615 (3636258) 3636258..3636830 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T220374 WP_001291435.1 NZ_CP085074:3631901-3632119 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T220374 NZ_CP117010:c2838502-2838251 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT220374 WP_000344800.1 NZ_CP085074:3631501-3631875 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT220374 NZ_CP117010:c2838254-2838000 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References