Detailed information of TA system TA220363

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1972294..1973129
• Replicon: chromosome
• Accession: NZ_CP085074
• Organism: Escherichia coli strain P21_SE1_04.20

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: LH372_RS09510
• Protein ID: WP_042046912.1
• Coordinates: 1972294..1972671 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: LH372_RS09515
• Protein ID: WP_073961352.1
• Coordinates: 1972761..1973129 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LH372_RS09490 (1969032)	1969032..1969778	-	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
LH372_RS09495 (1969793)	1969793..1971334	-	1542	WP_002431311.1	IS21-like element ISEc12 family transposase	-
LH372_RS09500 (1971968)	1971968..1972048	-	81	Protein_1864	hypothetical protein	-
LH372_RS09505 (1972148)	1972148..1972297	-	150	Protein_1865	DUF5983 family protein	-
LH372_RS09510 (1972294)	1972294..1972671	-	378	WP_042046912.1	TA system toxin CbtA family protein	Toxin
LH372_RS09515 (1972761)	1972761..1973129	-	369	WP_073961352.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LH372_RS09520 (1973292)	1973292..1973513	-	222	WP_000692341.1	DUF987 domain-containing protein	-
LH372_RS09525 (1973576)	1973576..1974052	-	477	WP_001186774.1	RadC family protein	-
LH372_RS09530 (1974068)	1974068..1974541	-	474	WP_226907394.1	antirestriction protein	-
LH372_RS09535 (1974883)	1974883..1975701	-	819	WP_106504503.1	DUF932 domain-containing protein	-
LH372_RS09540 (1975819)	1975819..1976014	-	196	Protein_1872	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	galF / cpsACP / wzi / wza / gmd / wcaG / wcaJ / gnd / manC / manB / ugd	1799111..2033559	234448

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14020.96 Da
• Isoelectric Point: 7.8276

>T220363 WP_042046912.1 NZ_CP085074:c1972671-1972294 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T220363 NZ_CP117010:834356-834763 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13444.17 Da
• Isoelectric Point: 6.2050

>AT220363 WP_073961352.1 NZ_CP085074:c1973129-1972761 [Escherichia coli]
VSDTLSGATHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT220363 NZ_CP117010:834109-834375 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA