Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 969900..970554 Replicon chromosome
Accession NZ_CP085074
Organism Escherichia coli strain P21_SE1_04.20

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag LH372_RS04725 Protein ID WP_000244777.1
Coordinates 970147..970554 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag LH372_RS04720 Protein ID WP_000354046.1
Coordinates 969900..970166 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LH372_RS04695 (965069) 965069..965812 + 744 WP_000951964.1 SDR family oxidoreductase -
LH372_RS04700 (965869) 965869..967302 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
LH372_RS04705 (967347) 967347..967658 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
LH372_RS04710 (967822) 967822..968481 + 660 WP_000250274.1 hemolysin III family protein -
LH372_RS04715 (968677) 968677..969657 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
LH372_RS04720 (969900) 969900..970166 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
LH372_RS04725 (970147) 970147..970554 + 408 WP_000244777.1 protein YgfX Toxin
LH372_RS04730 (970594) 970594..971115 - 522 WP_001055874.1 flavodoxin FldB -
LH372_RS04735 (971227) 971227..972123 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LH372_RS04740 (972148) 972148..972858 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LH372_RS04745 (972864) 972864..974597 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T220360 WP_000244777.1 NZ_CP085074:970147-970554 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T220360 NZ_CP117010:198750-198857 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT220360 WP_000354046.1 NZ_CP085074:969900-970166 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT220360 NZ_CP117010:c198702-198636 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References