Detailed information of TA system TA220345

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4275669..4276483
• Replicon: chromosome
• Accession: NZ_CP085069
• Organism: Escherichia coli strain P27_L1_05.20

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: LH371_RS20650
• Protein ID: WP_001054376.1
• Coordinates: 4275669..4275926 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: LH371_RS20655
• Protein ID: WP_001309181.1
• Coordinates: 4275938..4276483 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LH371_RS20625 (4270957)	4270957..4272063	+	1107	WP_001309184.1	N-acetylneuraminate epimerase	-
LH371_RS20630 (4272128)	4272128..4273108	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
LH371_RS20635 (4273218)	4273218..4273423	+	206	Protein_4029	HNH endonuclease	-
LH371_RS20640 (4273691)	4273691..4274931	-	1241	Protein_4030	helicase YjhR	-
LH371_RS20645 (4275047)	4275047..4275178	+	132	WP_001309182.1	hypothetical protein	-
LH371_RS20650 (4275669)	4275669..4275926	+	258	WP_001054376.1	YjhX family toxin	Toxin
LH371_RS20655 (4275938)	4275938..4276483	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
LH371_RS20660 (4276539)	4276539..4277285	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
LH371_RS20665 (4277454)	4277454..4277672	+	219	Protein_4035	hypothetical protein	-
LH371_RS20670 (4277710)	4277710..4277826	+	117	Protein_4036	VOC family protein	-
LH371_RS20675 (4278071)	4278071..4279192	+	1122	WP_000010829.1	M42 family metallopeptidase	-
LH371_RS20680 (4279189)	4279189..4279467	+	279	WP_000722973.1	PTS sugar transporter subunit IIB SgcB	-
LH371_RS20685 (4279479)	4279479..4280792	+	1314	WP_000460843.1	PTS sugar transporter subunit IIC SgcC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimI / fimA / fimE / fimB	4265455..4318024	52569

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T220345 WP_001054376.1 NZ_CP085069:4275669-4275926 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T220345 NZ_CP117008:2435635-2435811 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTCAG
GTTTCACGGGAGGCGCAGTGTCATGCCGCGTCACCCCAGCGAAGAGATTAAAGAACCATTGCGTAAAGCCATTCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT220345 WP_001309181.1 NZ_CP085069:4275938-4276483 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT220345 NZ_CP117008:2435857-2436273 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATCAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8