Detailed information of TA system TA220343

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3697538..3698375
• Replicon: chromosome
• Accession: NZ_CP085069
• Organism: Escherichia coli strain P27_L1_05.20

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: LH371_RS18005
• Protein ID: WP_000227784.1
• Coordinates: 3697833..3698375 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: LH371_RS18000
• Protein ID: WP_001297137.1
• Coordinates: 3697538..3697849 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LH371_RS17975 (3692558)	3692558..3693505	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
LH371_RS17980 (3693527)	3693527..3695518	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
LH371_RS17985 (3695508)	3695508..3696122	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
LH371_RS17990 (3696122)	3696122..3696451	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
LH371_RS17995 (3696463)	3696463..3697353	+	891	WP_000971336.1	heme o synthase	-
LH371_RS18000 (3697538)	3697538..3697849	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
LH371_RS18005 (3697833)	3697833..3698375	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
LH371_RS18010 (3698431)	3698431..3699366	-	936	WP_001297127.1	tetratricopeptide repeat protein	-
LH371_RS18015 (3699774)	3699774..3701138	+	1365	WP_001000978.1	MFS transporter	-
LH371_RS18020 (3701266)	3701266..3701757	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
LH371_RS18025 (3701925)	3701925..3702836	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T220343 WP_000227784.1 NZ_CP085069:3697833-3698375 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T220343 NZ_CP117008:1982294-1982548 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT220343 WP_001297137.1 NZ_CP085069:3697538-3697849 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT220343 NZ_CP117008:1982046-1982297 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3