220342

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3663460..3664078 Replicon chromosome
Accession NZ_CP085069
Organism Escherichia coli strain P27_L1_05.20

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LH371_RS17835 Protein ID WP_001291435.1
Coordinates 3663860..3664078 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LH371_RS17830 Protein ID WP_000344800.1
Coordinates 3663460..3663834 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LH371_RS17820 (3658549) 3658549..3659742 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LH371_RS17825 (3659765) 3659765..3662914 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
LH371_RS17830 (3663460) 3663460..3663834 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LH371_RS17835 (3663860) 3663860..3664078 + 219 WP_001291435.1 HHA domain-containing protein Toxin
LH371_RS17840 (3664250) 3664250..3664801 + 552 WP_000102568.1 maltose O-acetyltransferase -
LH371_RS17845 (3664917) 3664917..3665387 + 471 WP_000136192.1 YlaC family protein -
LH371_RS17850 (3665551) 3665551..3667101 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LH371_RS17855 (3667143) 3667143..3667496 - 354 WP_000878140.1 DUF1428 domain-containing protein -
LH371_RS17865 (3667875) 3667875..3668186 + 312 WP_000409911.1 MGMT family protein -
LH371_RS17870 (3668217) 3668217..3668789 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T220342 WP_001291435.1 NZ_CP085069:3663860-3664078 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T220342 NZ_CP117008:1982046-1982297 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT220342 WP_000344800.1 NZ_CP085069:3663460-3663834 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT220342 NZ_CP117008:1982294-1982548 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References