Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 866068..866722 Replicon chromosome
Accession NZ_CP085069
Organism Escherichia coli strain P27_L1_05.20

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag LH371_RS04230 Protein ID WP_000244781.1
Coordinates 866315..866722 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag LH371_RS04225 Protein ID WP_000354046.1
Coordinates 866068..866334 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LH371_RS04200 (861237) 861237..861980 + 744 WP_000951951.1 SDR family oxidoreductase -
LH371_RS04205 (862037) 862037..863470 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
LH371_RS04210 (863515) 863515..863826 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
LH371_RS04215 (863990) 863990..864649 + 660 WP_000250274.1 hemolysin III family protein -
LH371_RS04220 (864845) 864845..865825 - 981 WP_000886095.1 tRNA-modifying protein YgfZ -
LH371_RS04225 (866068) 866068..866334 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
LH371_RS04230 (866315) 866315..866722 + 408 WP_000244781.1 protein YgfX Toxin
LH371_RS04235 (866762) 866762..867283 - 522 WP_001055874.1 flavodoxin FldB -
LH371_RS04240 (867395) 867395..868291 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LH371_RS04245 (868316) 868316..869026 + 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LH371_RS04250 (869032) 869032..870765 + 1734 WP_000813218.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T220328 WP_000244781.1 NZ_CP085069:866315-866722 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T220328 NZ_CP117006:c4691395-4691084 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT220328 WP_000354046.1 NZ_CP085069:866068-866334 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT220328 NZ_CP117006:c4691083-4690793 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References