Detailed information of TA system TA220325

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 343204..344004
• Replicon: chromosome
• Accession: NZ_CP085069
• Organism: Escherichia coli strain P27_L1_05.20

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: F4NNI0
• Locus tag: LH371_RS01560
• Protein ID: WP_000342449.1
• Coordinates: 343477..344004 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: F4NNI1
• Locus tag: LH371_RS01555
• Protein ID: WP_001277108.1
• Coordinates: 343204..343470 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LH371_RS01535 (338862)	338862..339530	+	669	WP_000617723.1	cell division ATP-binding protein FtsE	-
LH371_RS01540 (339523)	339523..340581	+	1059	WP_096845886.1	permease-like cell division protein FtsX	-
LH371_RS01545 (340826)	340826..341680	+	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
LH371_RS01550 (341951)	341951..343054	+	1104	WP_001021996.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
LH371_RS01555 (343204)	343204..343470	+	267	WP_001277108.1	type II toxin-antitoxin system antitoxin AtaR	Antitoxin
LH371_RS01560 (343477)	343477..344004	+	528	WP_000342449.1	type II toxin-antitoxin system toxin acetyltransferase AtaT	Toxin
LH371_RS01565 (344001)	344001..344384	-	384	WP_000778785.1	aspartate 1-decarboxylase autocleavage activator PanM	-
LH371_RS01570 (344808)	344808..345917	+	1110	WP_096957216.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
LH371_RS01575 (345965)	345965..346891	+	927	WP_001295111.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
LH371_RS01580 (346888)	346888..348165	+	1278	WP_000803782.1	branched chain amino acid ABC transporter permease LivM	-
LH371_RS01585 (348162)	348162..348929	+	768	WP_000082110.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19691.70 Da
• Isoelectric Point: 7.7457

>T220325 WP_000342449.1 NZ_CP085069:343477-344004 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Nucleotide

• Length: 528 bp

>T220325 NZ_CP117006:c3870219-3869821 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9910.31 Da
• Isoelectric Point: 10.0863

>AT220325 WP_001277108.1 NZ_CP085069:343204-343470 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT220325 NZ_CP117006:c3870515-3870222 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCAAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAACTGGTGAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A829CLZ4

Antitoxin

• AlphaFold DB: A0A829CN24