Detailed information of TA system TA220323

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 8886..9375
• Replicon: plasmid unnamed2
• Accession: NZ_CP085066
• Organism: Escherichia coli strain P31_YH1_02.21

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U3PL64
• Locus tag: LH370_RS26350
• Protein ID: WP_020314676.1
• Coordinates: 8886..9146 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: LH370_RS26355
• Protein ID: WP_022540736.1
• Coordinates: 9136..9375 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LH370_RS26310 (LH370_26310)	4973..5302	+	330	WP_001542022.1	hypothetical protein	-
LH370_RS26315 (LH370_26315)	5706..6221	+	516	WP_001025393.1	J domain-containing protein	-
LH370_RS26320 (LH370_26320)	6325..6867	+	543	WP_001083904.1	hypothetical protein	-
LH370_RS26325 (LH370_26325)	6864..7118	+	255	WP_001230709.1	hypothetical protein	-
LH370_RS26330 (LH370_26330)	7115..7357	+	243	WP_000151456.1	hypothetical protein	-
LH370_RS26335 (LH370_26335)	7938..8261	+	324	WP_020314677.1	hypothetical protein	-
LH370_RS26340 (LH370_26340)	8304..8513	+	210	WP_020314678.1	hypothetical protein	-
LH370_RS26345 (LH370_26345)	8610..8834	+	225	WP_000814381.1	hypothetical protein	-
LH370_RS26350 (LH370_26350)	8886..9146	+	261	WP_020314676.1	hypothetical protein	Toxin
LH370_RS26355 (LH370_26355)	9136..9375	+	240	WP_022540736.1	transcriptional regulator	Antitoxin
LH370_RS26360 (LH370_26360)	9910..10170	+	261	WP_020314977.1	hypothetical protein	-
LH370_RS26365 (LH370_26365)	10557..11000	-	444	WP_022540743.1	hypothetical protein	-
LH370_RS26370 (LH370_26370)	11284..11466	+	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
LH370_RS26375 (LH370_26375)	11491..11928	+	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-
LH370_RS26380 (LH370_26380)	12059..12379	-	321	WP_001310435.1	hypothetical protein	-
LH370_RS26385 (LH370_26385)	12713..12891	-	179	Protein_22	hypothetical protein	-
LH370_RS26390 (LH370_26390)	12928..13191	-	264	WP_000224227.1	hypothetical protein	-
LH370_RS26395 (LH370_26395)	13493..13612	-	120	Protein_24	hypothetical protein	-
LH370_RS26400 (LH370_26400)	13632..14339	-	708	WP_226913213.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..62390	62390

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9922.30 Da
• Isoelectric Point: 7.2028

>T220323 WP_020314676.1 NZ_CP085066:8886-9146 [Escherichia coli]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEKAFDTIDFASDGLRDNDKEIVFMMIQEMS
EEENAN

Nucleotide

• Length: 261 bp

>T220323 NZ_CP117006:3610697-3610915 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9072.61 Da
• Isoelectric Point: 10.2613

>AT220323 WP_022540736.1 NZ_CP085066:9136-9375 [Escherichia coli]
MLTENTPENIKRLRKKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKYLLLLAGEHPEYVLSKRDRGILK

Nucleotide

• Length: 240 bp

>AT220323 NZ_CP117006:3610297-3610671 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A376MG86