220322

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 6864..7357 Replicon plasmid unnamed2
Accession NZ_CP085066
Organism Escherichia coli strain P31_YH1_02.21

Toxin (Protein)


Gene name TsxA Uniprot ID U9Y9W8
Locus tag LH370_RS26325 Protein ID WP_001230709.1
Coordinates 6864..7118 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID U9Y4R8
Locus tag LH370_RS26330 Protein ID WP_000151456.1
Coordinates 7115..7357 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LH370_RS26280 (LH370_26280) 2083..2244 + 162 WP_001128311.1 hypothetical protein -
LH370_RS26285 (LH370_26285) 2380..2532 + 153 Protein_2 FlmC family protein -
LH370_RS26290 (LH370_26290) 2480..2599 + 120 WP_227525580.1 Hok/Gef family protein -
LH370_RS26295 (LH370_26295) 2749..3345 + 597 WP_000125164.1 ProQ/FINO family protein -
LH370_RS26300 (LH370_26300) 3363..3647 - 285 WP_001162371.1 hypothetical protein -
LH370_RS26305 (LH370_26305) 3721..4386 - 666 WP_000532148.1 division plane positioning ATPase MipZ -
LH370_RS26310 (LH370_26310) 4973..5302 + 330 WP_001542022.1 hypothetical protein -
LH370_RS26315 (LH370_26315) 5706..6221 + 516 WP_001025393.1 J domain-containing protein -
LH370_RS26320 (LH370_26320) 6325..6867 + 543 WP_001083904.1 hypothetical protein -
LH370_RS26325 (LH370_26325) 6864..7118 + 255 WP_001230709.1 hypothetical protein Toxin
LH370_RS26330 (LH370_26330) 7115..7357 + 243 WP_000151456.1 hypothetical protein Antitoxin
LH370_RS26335 (LH370_26335) 7938..8261 + 324 WP_020314677.1 hypothetical protein -
LH370_RS26340 (LH370_26340) 8304..8513 + 210 WP_020314678.1 hypothetical protein -
LH370_RS26345 (LH370_26345) 8610..8834 + 225 WP_000814381.1 hypothetical protein -
LH370_RS26350 (LH370_26350) 8886..9146 + 261 WP_020314676.1 hypothetical protein -
LH370_RS26355 (LH370_26355) 9136..9375 + 240 WP_022540736.1 transcriptional regulator -
LH370_RS26360 (LH370_26360) 9910..10170 + 261 WP_020314977.1 hypothetical protein -
LH370_RS26365 (LH370_26365) 10557..11000 - 444 WP_022540743.1 hypothetical protein -
LH370_RS26370 (LH370_26370) 11284..11466 + 183 WP_000833470.1 type II toxin-antitoxin system HicA family toxin -
LH370_RS26375 (LH370_26375) 11491..11928 + 438 WP_000466318.1 type II toxin-antitoxin system HicB family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..62390 62390


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(1-124)

Antitoxin

Cro/C1-type helix-turn-helix domain

(53-94)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9942.47 Da        Isoelectric Point: 10.1206

>T220322 WP_001230709.1 NZ_CP085066:6864-7118 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Download         Length: 255 bp

>T220322 NZ_CP117006:3151288-3151674 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9136.45 Da        Isoelectric Point: 7.2775

>AT220322 WP_000151456.1 NZ_CP085066:7115-7357 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Download         Length: 243 bp

>AT220322 NZ_CP117006:3151664-3151993 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGAAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9H8F0

Antitoxin

Source ID Structure
AlphaFold DB A0A3G9GNF5

References