Detailed information of TA system TA220321

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 59032..59700
• Replicon: plasmid unnamed1
• Accession: NZ_CP085065
• Organism: Escherichia coli strain P31_YH1_02.21

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: LH370_RS26095
• Protein ID: WP_121372117.1
• Coordinates: 59032..59238 (+)
• Length: 69 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LH370_RS26100
• Protein ID: WP_121372118.1
• Coordinates: 59269..59700 (+)
• Length: 144 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LH370_RS26080 (LH370_26080)	54520..56202	-	1683	WP_000201469.1	portal protein	-
LH370_RS26085 (LH370_26085)	56267..57652	-	1386	WP_121372115.1	hypothetical protein	-
LH370_RS26090 (LH370_26090)	57859..59028	+	1170	WP_121372116.1	RNA-guided endonuclease TnpB family protein	-
LH370_RS26095 (LH370_26095)	59032..59238	+	207	WP_121372117.1	type II toxin-antitoxin system HicA family toxin	Toxin
LH370_RS26100 (LH370_26100)	59269..59700	+	432	WP_121372118.1	hypothetical protein	Antitoxin
LH370_RS26105 (LH370_26105)	60016..60474	+	459	WP_223363313.1	helix-turn-helix transcriptional regulator	-
LH370_RS26110 (LH370_26110)	60492..61244	+	753	WP_121372120.1	DUF3944 domain-containing protein	-
LH370_RS26115 (LH370_26115)	61275..61643	-	369	WP_016262342.1	hypothetical protein	-
LH370_RS26120 (LH370_26120)	61640..63592	-	1953	WP_121372121.1	head protein	-
LH370_RS26125 (LH370_26125)	63589..64206	-	618	WP_021551755.1	hypothetical protein	-
LH370_RS26130 (LH370_26130)	64196..64642	-	447	WP_016262345.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..94191	94191
	-	flank	IS/Tn	-	-	57859..59028	1169

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (13-65)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-82)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7671.83 Da
• Isoelectric Point: 11.2092

>T220321 WP_121372117.1 NZ_CP085065:59032-59238 [Escherichia coli]
VGSLARQGGEQSQFKRWLIQQGAEFRKAPGGGSHQKVNLNGKRSVFPDHGSKEMPEPLRKKIMKDLWL

Nucleotide

• Length: 207 bp

>T220321 NZ_CP117006:2897702-2898196 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin

Protein

• Length: 144 a.a.
• Molecular weight: 15641.98 Da
• Isoelectric Point: 5.2844

>AT220321 WP_121372118.1 NZ_CP085065:59269-59700 [Escherichia coli]
MFNYPVKLERDDKTGAYVVSCRDLPLFNSVGDSLEEALLEAGYGLVAAVAIEIEERRPVPTGSEPKEGEYVVSLPVLPAM
KAALHNAMIETGTRKAELARKLGKNGTQIDRLLDVEHSSKVETVELALHQLNKNIAVSVTPMR

Nucleotide

• Length: 432 bp

>AT220321 NZ_CP117006:2897412-2897705 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAACCAGGCGCGAATCTTTAAATTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA