Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | phd-doc/Doc-Phd |
| Location | 26354..26955 | Replicon | plasmid unnamed1 |
| Accession | NZ_CP085065 | ||
| Organism | Escherichia coli strain P31_YH1_02.21 | ||
Toxin (Protein)
| Gene name | doc | Uniprot ID | A0A829IWB6 |
| Locus tag | LH370_RS25935 | Protein ID | WP_016262419.1 |
| Coordinates | 26575..26955 (+) | Length | 127 a.a. |
Antitoxin (Protein)
| Gene name | phd | Uniprot ID | U9YQH9 |
| Locus tag | LH370_RS25930 | Protein ID | WP_001190712.1 |
| Coordinates | 26354..26575 (+) | Length | 74 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LH370_RS25920 (LH370_25920) | 23329..24618 | - | 1290 | WP_121372111.1 | restriction endonuclease subunit S | - |
| LH370_RS25925 (LH370_25925) | 24615..26171 | - | 1557 | WP_029701840.1 | type I restriction-modification system subunit M | - |
| LH370_RS25930 (LH370_25930) | 26354..26575 | + | 222 | WP_001190712.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
| LH370_RS25935 (LH370_25935) | 26575..26955 | + | 381 | WP_016262419.1 | type II toxin-antitoxin system death-on-curing family toxin | Toxin |
| LH370_RS25940 (LH370_25940) | 27036..27833 | - | 798 | WP_016262420.1 | hypothetical protein | - |
| LH370_RS25945 (LH370_25945) | 27840..28490 | - | 651 | WP_021551780.1 | DUF2829 domain-containing protein | - |
| LH370_RS25950 (LH370_25950) | 28505..28999 | - | 495 | WP_097430097.1 | dUTP diphosphatase | - |
| LH370_RS25955 (LH370_25955) | 28996..29484 | - | 489 | WP_016262423.1 | hypothetical protein | - |
| LH370_RS25960 (LH370_25960) | 29481..29825 | - | 345 | WP_016262424.1 | hypothetical protein | - |
| LH370_RS25965 (LH370_25965) | 29898..30926 | - | 1029 | WP_021551777.1 | tyrosine-type recombinase/integrase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..94191 | 94191 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 127 a.a. Molecular weight: 13678.55 Da Isoelectric Point: 5.6406
>T220320 WP_016262419.1 NZ_CP085065:26575-26955 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGIQVYDSPVLVELAVGAATGEIPVSSVAEKLRELYGSNI
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGIQVYDSPVLVELAVGAATGEIPVSSVAEKLRELYGSNI
Download Length: 381 bp
>T220320 NZ_CP117006:2769660-2769767 [Escherichia coli]
ATGACGCTCACGCAGTTTGCCATGACTTTCTGGCACGACTTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA
ATGACGCTCACGCAGTTTGCCATGACTTTCTGGCACGACTTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA
Antitoxin
Download Length: 74 a.a. Molecular weight: 8133.10 Da Isoelectric Point: 4.7708
>AT220320 WP_001190712.1 NZ_CP085065:26354-26575 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR
Download Length: 222 bp
>AT220320 NZ_CP117006:c2769610-2769547 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTATACCTCTCAACGTGCGGGGGTTTTCT
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTATACCTCTCAACGTGCGGGGGTTTTCT
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829IWB6 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829CJB6 |