Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4246873..4247527 Replicon chromosome
Accession NZ_CP085063
Organism Escherichia coli strain P31_YH1_02.21

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag LH370_RS20485 Protein ID WP_000244781.1
Coordinates 4247120..4247527 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag LH370_RS20480 Protein ID WP_000354046.1
Coordinates 4246873..4247139 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LH370_RS20460 (4242961) 4242961..4244394 - 1434 WP_001394742.1 6-phospho-beta-glucosidase BglA -
LH370_RS20465 (4244439) 4244439..4244750 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
LH370_RS20470 (4244914) 4244914..4245573 + 660 WP_000250274.1 hemolysin III family protein -
LH370_RS20475 (4245650) 4245650..4246630 - 981 WP_000886087.1 tRNA-modifying protein YgfZ -
LH370_RS20480 (4246873) 4246873..4247139 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
LH370_RS20485 (4247120) 4247120..4247527 + 408 WP_000244781.1 protein YgfX Toxin
LH370_RS20490 (4247567) 4247567..4248088 - 522 WP_001055874.1 flavodoxin FldB -
LH370_RS20495 (4248200) 4248200..4249096 + 897 WP_000806650.1 site-specific tyrosine recombinase XerD -
LH370_RS20500 (4249121) 4249121..4249831 + 711 WP_000715222.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LH370_RS20505 (4249837) 4249837..4251570 + 1734 WP_000813233.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T220314 WP_000244781.1 NZ_CP085063:4247120-4247527 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T220314 NZ_CP117006:1869623-1869877 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT220314 WP_000354046.1 NZ_CP085063:4246873-4247139 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT220314 NZ_CP117006:1869375-1869626 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References