Detailed information of TA system TA220308

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 2629989..2630584
• Replicon: chromosome
• Accession: NZ_CP085063
• Organism: Escherichia coli strain P31_YH1_02.21

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: LH370_RS12810
• Protein ID: WP_000239581.1
• Coordinates: 2629989..2630339 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: LH370_RS12815
• Protein ID: WP_001223213.1
• Coordinates: 2630333..2630584 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LH370_RS12790 (2625443)	2625443..2626465	-	1023	WP_001361374.1	ABC transporter permease	-
LH370_RS12795 (2626479)	2626479..2627981	-	1503	WP_001522399.1	sugar ABC transporter ATP-binding protein	-
LH370_RS12800 (2628114)	2628114..2629070	-	957	WP_001522398.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
LH370_RS12805 (2629380)	2629380..2629910	+	531	WP_000055075.1	inorganic diphosphatase	-
LH370_RS12810 (2629989)	2629989..2630339	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
LH370_RS12815 (2630333)	2630333..2630584	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
LH370_RS12820 (2630796)	2630796..2631137	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
LH370_RS12825 (2631140)	2631140..2634919	-	3780	WP_001522396.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T220308 WP_000239581.1 NZ_CP085063:c2630339-2629989 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T220308 NZ_CP117006:c632393-631929 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTACAGCGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAGCCCATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT220308 WP_001223213.1 NZ_CP085063:c2630584-2630333 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT220308 NZ_CP117006:c632728-632393 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACACTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7