Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1897417..1898035 Replicon chromosome
Accession NZ_CP085063
Organism Escherichia coli strain P31_YH1_02.21

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LH370_RS09325 Protein ID WP_001291435.1
Coordinates 1897817..1898035 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LH370_RS09320 Protein ID WP_000344800.1
Coordinates 1897417..1897791 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LH370_RS09310 (1892506) 1892506..1893699 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LH370_RS09315 (1893722) 1893722..1896871 + 3150 WP_001132480.1 efflux RND transporter permease AcrB -
LH370_RS09320 (1897417) 1897417..1897791 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LH370_RS09325 (1897817) 1897817..1898035 + 219 WP_001291435.1 HHA domain-containing protein Toxin
LH370_RS09330 (1898207) 1898207..1898758 + 552 WP_001522034.1 maltose O-acetyltransferase -
LH370_RS09335 (1898874) 1898874..1899344 + 471 WP_001490888.1 YlaC family protein -
LH370_RS09340 (1899508) 1899508..1901058 + 1551 WP_001577872.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LH370_RS09345 (1901100) 1901100..1901453 - 354 WP_000878140.1 DUF1428 domain-containing protein -
LH370_RS09355 (1901832) 1901832..1902143 + 312 WP_000409911.1 MGMT family protein -
LH370_RS09360 (1902174) 1902174..1902746 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T220303 WP_001291435.1 NZ_CP085063:1897817-1898035 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T220303 NZ_CP117004:c42842-42693 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACTGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT220303 WP_000344800.1 NZ_CP085063:1897417-1897791 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT220303 NZ_CP117004:42886-42947 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References