Detailed information of TA system TA220302

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 1473600..1474305
• Replicon: chromosome
• Accession: NZ_CP085063
• Organism: Escherichia coli strain P31_YH1_02.21

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: LH370_RS07450
• Protein ID: WP_000539521.1
• Coordinates: 1473600..1473986 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LH370_RS07455
• Protein ID: WP_001280945.1
• Coordinates: 1473976..1474305 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LH370_RS07430 (1469604)	1469604..1470230	+	627	WP_020233670.1	glutathione S-transferase GstB	-
LH370_RS07435 (1470227)	1470227..1471342	-	1116	WP_000555050.1	aldose sugar dehydrogenase YliI	-
LH370_RS07440 (1471453)	1471453..1471836	-	384	WP_000497137.1	biofilm formation regulator BssR	-
LH370_RS07445 (1472049)	1472049..1473374	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
LH370_RS07450 (1473600)	1473600..1473986	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LH370_RS07455 (1473976)	1473976..1474305	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
LH370_RS07460 (1474375)	1474375..1475703	-	1329	WP_000086873.1	GGDEF domain-containing protein	-
LH370_RS07465 (1475711)	1475711..1478059	-	2349	WP_021523051.1	EAL domain-containing protein	-
LH370_RS07470 (1478237)	1478237..1479148	-	912	WP_001236019.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T220302 WP_000539521.1 NZ_CP085063:1473600-1473986 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T220302 NZ_CP117004:c39813-39535 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGT
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACTGTCGTG
TGGAAGGTGCCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGATAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGTGTATTGCATACTACACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT220302 WP_001280945.1 NZ_CP085063:1473976-1474305 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT220302 NZ_CP117004:c40082-39813 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCAAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCTGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6