Detailed information of TA system TA220301

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1217229..1218013
• Replicon: chromosome
• Accession: NZ_CP085063
• Organism: Escherichia coli strain P31_YH1_02.21

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: LH370_RS06240
• Protein ID: WP_000613626.1
• Coordinates: 1217519..1218013 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: LH370_RS06235
• Protein ID: WP_001110447.1
• Coordinates: 1217229..1217522 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LH370_RS06225 (1212379)	1212379..1213338	-	960	WP_023153797.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
LH370_RS06230 (1213911)	1213911..1217096	+	3186	WP_001522867.1	ribonuclease E	-
LH370_RS06235 (1217229)	1217229..1217522	+	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
LH370_RS06240 (1217519)	1217519..1218013	+	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
LH370_RS06245 (1218108)	1218108..1219061	-	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
LH370_RS06250 (1219073)	1219073..1220716	-	1644	WP_001522865.1	flagellar hook-associated protein FlgK	-
LH370_RS06255 (1220782)	1220782..1221723	-	942	WP_001522863.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
LH370_RS06260 (1221723)	1221723..1222820	-	1098	WP_000589326.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T220301 WP_000613626.1 NZ_CP085063:1217519-1218013 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T220301 NZ_CP117003:26658-26936 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCATCCCCATTTTGTTCTGGTTTATGAAGTCGATAGCCAGTGGGGTAAAGTGTAT
ATCCTGCGCGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT220301 WP_001110447.1 NZ_CP085063:1217229-1217522 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT220301 NZ_CP117003:26389-26658 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCGTGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCTGCCGCTGCATGGCGGGAT
GAAATGCGCAGGAAGGTTGCCGATAAATGA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6