Detailed information of TA system TA220300

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 778328..778966
• Replicon: chromosome
• Accession: NZ_CP085063
• Organism: Escherichia coli strain P31_YH1_02.21

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0D8WF76
• Locus tag: LH370_RS03925
• Protein ID: WP_001306887.1
• Coordinates: 778790..778966 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LH370_RS03920
• Protein ID: WP_001270286.1
• Coordinates: 778328..778744 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LH370_RS03900 (773480)	773480..774421	-	942	WP_001251315.1	ABC transporter permease	-
LH370_RS03905 (774422)	774422..775435	-	1014	WP_001523231.1	ABC transporter ATP-binding protein	-
LH370_RS03910 (775453)	775453..776598	-	1146	WP_020233879.1	ABC transporter substrate-binding protein	-
LH370_RS03915 (776843)	776843..778249	-	1407	WP_001523229.1	PLP-dependent aminotransferase family protein	-
LH370_RS03920 (778328)	778328..778744	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
LH370_RS03925 (778790)	778790..778966	-	177	WP_001306887.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
LH370_RS03930 (779188)	779188..779418	+	231	WP_000494241.1	YncJ family protein	-
LH370_RS03935 (779510)	779510..781471	-	1962	WP_057950830.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
LH370_RS03940 (781544)	781544..782080	-	537	WP_020233877.1	DNA-binding transcriptional regulator SutR	-
LH370_RS03945 (782172)	782172..783344	+	1173	WP_001523225.1	BenE family transporter YdcO	-
LH370_RS03950 (783452)	783452..783910	-	459	WP_000526135.1	IS200/IS605-like element IS200C family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	783452..783910	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6768.88 Da
• Isoelectric Point: 11.5336

>T220300 WP_001306887.1 NZ_CP085063:c778966-778790 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFRGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T220300 NZ_CP117002:33851-34372 [Escherichia coli]
GTGAGCAATACGACGATAGAGATTTTCTCTGGAGAGAAAGATTATGATCTAAACGGTTTTGATTGCGGCGAAGAGTCACT
AAACGCCTTTTTAGCCAACCACTTAAAAAGGCAGCATGAGGGAAAAATTCTTCGTGCTTATGTGCTTTGCACTCAAGAAG
AAAGGCCAAAAGTGTTAGGGTATTACACTTTGTCAGGTAGTTGTTTTGAAAAGGAGTCCTTACCTTCACGAAGCAAACAA
AAGAAGGTTCCTTATCGGAATGTTCCAAGTGTTACTTTGGGTAGGCTGGCTTTGGACAAGTCCCTTCAAGGTCAAGGATT
TGGCTCAATGCTTGTAACACATGCAATGCGCGTTGTGTACAATGCATCTCTTGCTGTAGGCATTCATGGACTTTTCGTTG
AGGCGTTAAATGACAAAGCCAAGGCGTTTTATAAAAGTTTAGGCTTTATCCAGCTGGTTGGTAACAACGAGCGTTCTTTG
TTCTATCCTACAAAATCTATCGAAAAATTGTTCGAAGAATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT220300 WP_001270286.1 NZ_CP085063:c778744-778328 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT220300 NZ_CP117002:33574-33843 [Escherichia coli]
ATGTCAGCACTAAAAAAGCAGCGCATCGATTTGAGATTAACCGATGACGATAAAAGCATCATCGAGGAAGCTGCCGCAAT
GTCTAACCAGAGCATTACTCAGTTTATGGTTAGCAGTGCATCTGAACGTGCCGTGAAAGTTATAGAGCAACACCGTAGAC
TAGTTCTGAATGAAGAATCCTGGAATCTGGTTATGGACGCTATAAGCAATCCTCCGGCACCGAACGATAAGCTGAAACGA
GCTGCTAATCGTCTGAAAAGCATGGAGTAG

Structures

Toxin

• AlphaFold DB: A0A0D8WF76