Detailed information of TA system TA220299

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 678081..679042
• Replicon: chromosome
• Accession: NZ_CP085063
• Organism: Escherichia coli strain P31_YH1_02.21

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A1Q5ZNB7
• Locus tag: LH370_RS03490
• Protein ID: WP_021523084.1
• Coordinates: 678347..679042 (+)
• Length: 232 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: LH370_RS03485
• Protein ID: WP_001296726.1
• Coordinates: 678081..678347 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LH370_RS03485 (678081)	678081..678347	+	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
LH370_RS03490 (678347)	678347..679042	+	696	WP_021523084.1	HipA N-terminal domain-containing protein	Toxin
LH370_RS03495 (679242)	679242..680594	-	1353	WP_001551127.1	SIR2 family protein	-
LH370_RS03500 (680809)	680809..681841	-	1033	Protein_687	ISNCY family transposase	-
LH370_RS03505 (682208)	682208..682474	-	267	Protein_688	LysR family transcriptional regulator	-
LH370_RS03510 (682478)	682478..683029	+	552	Protein_689	aldo/keto reductase	-
LH370_RS03515 (683132)	683132..683287	+	156	WP_001322415.1	hypothetical protein	-
LH370_RS03520 (683247)	683247..683810	+	564	WP_023153757.1	amidohydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	680809..681627	818

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 232 a.a.
• Molecular weight: 25773.79 Da
• Isoelectric Point: 9.9928

>T220299 WP_021523084.1 NZ_CP085063:678347-679042 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETIQAGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGVNASKGKKTAIDKI
YPRHFLATAKVLKFPEVQMHEILSDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 696 bp

>T220299 NZ_CP117001:c4267176-4266958 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT220299 WP_001296726.1 NZ_CP085063:678081-678347 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT220299 NZ_CP117001:c4267576-4267202 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A1Q5ZNB7