Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 148550..149075 Replicon plasmid pNDM_P30_L1_05.20
Accession NZ_CP085061
Organism Escherichia coli strain P30_L1_05.20

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag LH369_RS24370 Protein ID WP_001159868.1
Coordinates 148550..148855 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag LH369_RS24375 Protein ID WP_000813634.1
Coordinates 148857..149075 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LH369_RS24355 (144460) 144460..145626 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
LH369_RS24360 (146214) 146214..146969 - 756 WP_000852146.1 replication initiation protein RepE -
LH369_RS24365 (147743) 147743..148549 - 807 WP_000016982.1 site-specific integrase -
LH369_RS24370 (148550) 148550..148855 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
LH369_RS24375 (148857) 148857..149075 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
LH369_RS24380 (149655) 149655..150743 + 1089 WP_033554392.1 transcriptional repressor PifC -
LH369_RS24385 (150745) 150745..152970 + 2226 WP_033554391.1 P-loop NTPase fold protein -
LH369_RS24390 (153020) 153020..153919 - 900 WP_032173297.1 nucleotide-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / rmtB / blaTEM-1B / mph(A) / erm(B) - 1..161701 161701


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T220284 WP_001159868.1 NZ_CP085061:c148855-148550 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T220284 NZ_CP117001:c1047248-1047145 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT220284 WP_000813634.1 NZ_CP085061:c149075-148857 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT220284 NZ_CP117001:1047107-1047378 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References