Detailed information of TA system TA220280

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 5195..5754
• Replicon: plasmid pNDM_P30_L1_05.20
• Accession: NZ_CP085061
• Organism: Escherichia coli strain P30_L1_05.20

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V0SVC8
• Locus tag: LH369_RS23495
• Protein ID: WP_000421272.1
• Coordinates: 5195..5470 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0SRR3
• Locus tag: LH369_RS23500
• Protein ID: WP_001387467.1
• Coordinates: 5470..5754 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LH369_RS23460 (1256)	1256..1996	-	741	WP_001066941.1	tyrosine-type recombinase/integrase	-
LH369_RS23465 (2117)	2117..2299	-	183	WP_050488504.1	hypothetical protein	-
LH369_RS23470 (2366)	2366..2656	-	291	Protein_3	type II toxin-antitoxin system RelE/ParE family toxin	-
LH369_RS23475 (2643)	2643..2899	-	257	Protein_4	type II toxin-antitoxin system ParD family antitoxin	-
LH369_RS23480 (3431)	3431..4600	+	1170	WP_001403369.1	hypothetical protein	-
LH369_RS23485 (4633)	4633..4761	+	129	Protein_6	IS481 family transposase	-
LH369_RS23490 (4886)	4886..5089	-	204	Protein_7	hypothetical protein	-
LH369_RS23495 (5195)	5195..5470	-	276	WP_000421272.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LH369_RS23500 (5470)	5470..5754	-	285	WP_001387467.1	ribbon-helix-helix domain-containing protein	Antitoxin
LH369_RS23505 (6359)	6359..7111	+	753	WP_001403368.1	IclR family transcriptional regulator	-
LH369_RS23510 (7157)	7157..8122	-	966	WP_001022265.1	2-keto-3-deoxygluconate transporter	-
LH369_RS23515 (8155)	8155..8535	-	381	WP_000710783.1	Rid family detoxifying hydrolase	-
LH369_RS23520 (8560)	8560..9450	-	891	WP_001077068.1	dihydrodipicolinate synthase family protein	-
LH369_RS23525 (9804)	9804..10370	+	567	Protein_14	zinc-binding dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / rmtB / blaTEM-1B / mph(A) / erm(B)	-	1..161701	161701

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 10.0871

>T220280 WP_000421272.1 NZ_CP085061:c5470-5195 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T220280 NZ_CP085061:c5470-5195 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10460.87 Da
• Isoelectric Point: 5.6558

>AT220280 WP_001387467.1 NZ_CP085061:c5754-5470 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT220280 NZ_CP085061:c5754-5470 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAGCAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: V0SVC8

Antitoxin

• AlphaFold DB: A0A0E0Y880