Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3708325..3708979 Replicon chromosome
Accession NZ_CP085060
Organism Escherichia coli strain P30_L1_05.20

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag LH369_RS17760 Protein ID WP_000244777.1
Coordinates 3708325..3708732 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag LH369_RS17765 Protein ID WP_000354046.1
Coordinates 3708713..3708979 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LH369_RS17740 (3704282) 3704282..3706015 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
LH369_RS17745 (3706021) 3706021..3706731 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LH369_RS17750 (3706756) 3706756..3707652 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LH369_RS17755 (3707764) 3707764..3708285 + 522 WP_001055874.1 flavodoxin FldB -
LH369_RS17760 (3708325) 3708325..3708732 - 408 WP_000244777.1 protein YgfX Toxin
LH369_RS17765 (3708713) 3708713..3708979 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
LH369_RS17770 (3709222) 3709222..3710202 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
LH369_RS17775 (3710398) 3710398..3711057 - 660 WP_000250274.1 hemolysin III family protein -
LH369_RS17780 (3711221) 3711221..3711532 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
LH369_RS17785 (3711577) 3711577..3713010 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
LH369_RS17790 (3713067) 3713067..3713810 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T220275 WP_000244777.1 NZ_CP085060:c3708732-3708325 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T220275 NZ_CP116995:129286-129435 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT220275 WP_000354046.1 NZ_CP085060:c3708979-3708713 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT220275 NZ_CP116995:c129242-129181 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References