Detailed information of TA system TA220272

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2767481..2768316
• Replicon: chromosome
• Accession: NZ_CP085060
• Organism: Escherichia coli strain P30_L1_05.20

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7I6H0K3
• Locus tag: LH369_RS13455
• Protein ID: WP_000854760.1
• Coordinates: 2767939..2768316 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7U9IW59
• Locus tag: LH369_RS13450
• Protein ID: WP_001360327.1
• Coordinates: 2767481..2767849 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LH369_RS13425 (2764596)	2764596..2764791	+	196	Protein_2619	DUF905 family protein	-
LH369_RS13430 (2764909)	2764909..2765727	+	819	WP_001234729.1	DUF932 domain-containing protein	-
LH369_RS13435 (2766069)	2766069..2766542	+	474	WP_000855059.1	antirestriction protein	-
LH369_RS13440 (2766558)	2766558..2767034	+	477	WP_133963824.1	RadC family protein	-
LH369_RS13445 (2767097)	2767097..2767318	+	222	WP_000692286.1	DUF987 domain-containing protein	-
LH369_RS13450 (2767481)	2767481..2767849	+	369	WP_001360327.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LH369_RS13455 (2767939)	2767939..2768316	+	378	WP_000854760.1	TA system toxin CbtA family protein	Toxin
LH369_RS13460 (2768313)	2768313..2768462	+	150	Protein_2626	DUF5983 family protein	-
LH369_RS13465 (2768562)	2768562..2768642	+	81	Protein_2627	hypothetical protein	-
LH369_RS13470 (2769458)	2769458..2769829	+	372	WP_001295631.1	IS110 family transposase	-
LH369_RS13475 (2770100)	2770100..2770465	+	366	WP_001280454.1	EutP/PduV family microcompartment system protein	-
LH369_RS13480 (2770566)	2770566..2770895	-	330	WP_000450409.1	DUF496 family protein	-
LH369_RS13485 (2771067)	2771067..2772125	-	1059	WP_001200895.1	FUSC family protein	-
LH369_RS13490 (2772323)	2772323..2772796	-	474	WP_001105407.1	DNA gyrase inhibitor SbmC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14165.19 Da
• Isoelectric Point: 7.3249

>T220272 WP_000854760.1 NZ_CP085060:2767939-2768316 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T220272 NZ_CP116994:c4266540-4266322 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13593.34 Da
• Isoelectric Point: 5.9598

>AT220272 WP_001360327.1 NZ_CP085060:2767481-2767849 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT220272 NZ_CP116994:c4266940-4266566 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A7I6H0K3

Antitoxin

• AlphaFold DB: A0A7U9IW59