Detailed information of TA system TA220262

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2205071..2205709
• Replicon: chromosome
• Accession: NZ_CP085060
• Organism: Escherichia coli strain P30_L1_05.20

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: LH369_RS10650
• Protein ID: WP_001447010.1
• Coordinates: 2205071..2205247 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LH369_RS10655
• Protein ID: WP_001270286.1
• Coordinates: 2205293..2205709 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LH369_RS10630 (2200690)	2200690..2201865	-	1176	WP_001236265.1	BenE family transporter YdcO	-
LH369_RS10635 (2201957)	2201957..2202493	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
LH369_RS10640 (2202566)	2202566..2204527	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
LH369_RS10645 (2204619)	2204619..2204849	-	231	WP_000494244.1	YncJ family protein	-
LH369_RS10650 (2205071)	2205071..2205247	+	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
LH369_RS10655 (2205293)	2205293..2205709	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
LH369_RS10660 (2205788)	2205788..2207194	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
LH369_RS10665 (2207439)	2207439..2208584	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
LH369_RS10670 (2208602)	2208602..2209615	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
LH369_RS10675 (2209616)	2209616..2210557	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T220262 WP_001447010.1 NZ_CP085060:2205071-2205247 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T220262 NZ_CP116994:c1983219-1982884 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT220262 WP_001270286.1 NZ_CP085060:2205293-2205709 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT220262 NZ_CP116994:c1983467-1983219 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8