Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1086493..1087111 Replicon chromosome
Accession NZ_CP085060
Organism Escherichia coli strain P30_L1_05.20

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LH369_RS05215 Protein ID WP_001291435.1
Coordinates 1086493..1086711 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LH369_RS05220 Protein ID WP_000344800.1
Coordinates 1086737..1087111 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LH369_RS05180 (1081782) 1081782..1082354 + 573 WP_000779831.1 YbaY family lipoprotein -
LH369_RS05185 (1082385) 1082385..1082696 - 312 WP_000409911.1 MGMT family protein -
LH369_RS05195 (1083075) 1083075..1083428 + 354 WP_000878140.1 DUF1428 domain-containing protein -
LH369_RS05200 (1083470) 1083470..1085020 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LH369_RS05205 (1085184) 1085184..1085654 - 471 WP_000136192.1 YlaC family protein -
LH369_RS05210 (1085770) 1085770..1086321 - 552 WP_000102564.1 maltose O-acetyltransferase -
LH369_RS05215 (1086493) 1086493..1086711 - 219 WP_001291435.1 HHA domain-containing protein Toxin
LH369_RS05220 (1086737) 1086737..1087111 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LH369_RS05225 (1087657) 1087657..1090806 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
LH369_RS05230 (1090829) 1090829..1092022 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T220260 WP_001291435.1 NZ_CP085060:c1086711-1086493 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T220260 NZ_CP116994:c1226744-1226493 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT220260 WP_000344800.1 NZ_CP085060:c1087111-1086737 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT220260 NZ_CP116994:c1226496-1226242 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References