Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 60910..61511 Replicon plasmid unnamed2
Accession NZ_CP085058
Organism Escherichia coli strain P24_WM1_05.20

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag LHK10_RS24595 Protein ID WP_001216045.1
Coordinates 60910..61290 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag LHK10_RS24600 Protein ID WP_001190712.1
Coordinates 61290..61511 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LHK10_RS24570 (LHK10_24570) 56351..57793 - 1443 WP_223656860.1 terminase -
LHK10_RS24575 (LHK10_24575) 57835..59028 - 1194 WP_226926687.1 terminase -
LHK10_RS24580 (LHK10_24580) 59114..59566 - 453 WP_050858701.1 Late promoter-activating protein -
LHK10_RS24585 (LHK10_24585) 59655..60698 - 1044 WP_047649471.1 DUF968 domain-containing protein -
LHK10_RS24590 (LHK10_24590) 60726..60905 - 180 WP_000113019.1 hypothetical protein -
LHK10_RS24595 (LHK10_24595) 60910..61290 - 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
LHK10_RS24600 (LHK10_24600) 61290..61511 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LHK10_RS24605 (LHK10_24605) 61584..61973 - 390 WP_000506730.1 S24 family peptidase -
LHK10_RS24610 (LHK10_24610) 62097..62348 - 252 WP_001377386.1 DNA polymerase III subunit theta -
LHK10_RS24615 (LHK10_24615) 62798..62935 + 138 WP_000123562.1 hypothetical protein -
LHK10_RS24620 (LHK10_24620) 63010..63372 - 363 WP_001261544.1 hypothetical protein -
LHK10_RS24625 (LHK10_24625) 63369..64301 - 933 WP_000057449.1 hypothetical protein -
LHK10_RS24630 (LHK10_24630) 64283..64657 - 375 WP_000988651.1 hypothetical protein -
LHK10_RS24635 (LHK10_24635) 64664..64957 - 294 WP_000268000.1 hypothetical protein -
LHK10_RS24640 (LHK10_24640) 65136..65369 - 234 WP_000517420.1 hypothetical protein -
LHK10_RS24645 (LHK10_24645) 65446..65706 - 261 WP_033553819.1 hypothetical protein -
LHK10_RS24650 (LHK10_24650) 65703..65978 - 276 Protein_67 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..92591 92591


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T220254 WP_001216045.1 NZ_CP085058:c61290-60910 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T220254 NZ_CP116994:595363-595539 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT220254 WP_001190712.1 NZ_CP085058:c61511-61290 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT220254 NZ_CP116994:595585-596001 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References