Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 96316..96959 Replicon plasmid unnamed1
Accession NZ_CP085057
Organism Escherichia coli strain P24_WM1_05.20

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag LHK10_RS24210 Protein ID WP_001034044.1
Coordinates 96543..96959 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag LHK10_RS24205 Protein ID WP_001261286.1
Coordinates 96316..96546 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LHK10_RS24185 (92947) 92947..93702 - 756 WP_000852146.1 replication initiation protein RepE -
LHK10_RS24190 (94424) 94424..95230 - 807 WP_000016970.1 site-specific integrase -
LHK10_RS24195 (95231) 95231..95536 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
LHK10_RS24200 (95538) 95538..95756 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
LHK10_RS24205 (96316) 96316..96546 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LHK10_RS24210 (96543) 96543..96959 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
LHK10_RS24215 (97034) 97034..98599 + 1566 WP_001128474.1 AAA family ATPase -
LHK10_RS24220 (98584) 98584..99606 + 1023 WP_000361402.1 helicase UvrD -
LHK10_RS24225 (99860) 99860..100546 - 687 Protein_116 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-15 / aac(6')-Ib-cr / blaOXA-1 / tet(A) / sitABCD senB / iutA / iucD / iucC / iucB / iucA 1..118805 118805
- flank IS/Tn - - 99860..100207 347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T220253 WP_001034044.1 NZ_CP085057:96543-96959 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T220253 NZ_CP116994:c332229-332122 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT220253 WP_001261286.1 NZ_CP085057:96316-96546 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT220253 NZ_CP116994:332276-332342 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References