Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | ccdAB/CcdA(antitoxin) |
| Location | 95231..95756 | Replicon | plasmid unnamed1 |
| Accession | NZ_CP085057 | ||
| Organism | Escherichia coli strain P24_WM1_05.20 | ||
Toxin (Protein)
| Gene name | ccdB | Uniprot ID | S1PFV8 |
| Locus tag | LHK10_RS24195 | Protein ID | WP_001159871.1 |
| Coordinates | 95231..95536 (-) | Length | 102 a.a. |
Antitoxin (Protein)
| Gene name | ccdA | Uniprot ID | H9TJP1 |
| Locus tag | LHK10_RS24200 | Protein ID | WP_000813630.1 |
| Coordinates | 95538..95756 (-) | Length | 73 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LHK10_RS24180 (91193) | 91193..92359 | - | 1167 | WP_000772446.1 | plasmid-partitioning protein SopA | - |
| LHK10_RS24185 (92947) | 92947..93702 | - | 756 | WP_000852146.1 | replication initiation protein RepE | - |
| LHK10_RS24190 (94424) | 94424..95230 | - | 807 | WP_000016970.1 | site-specific integrase | - |
| LHK10_RS24195 (95231) | 95231..95536 | - | 306 | WP_001159871.1 | type II toxin-antitoxin system toxin CcdB | Toxin |
| LHK10_RS24200 (95538) | 95538..95756 | - | 219 | WP_000813630.1 | type II toxin-antitoxin system antitoxin CcdA | Antitoxin |
| LHK10_RS24205 (96316) | 96316..96546 | + | 231 | WP_001261286.1 | type II toxin-antitoxin system VapB family antitoxin | - |
| LHK10_RS24210 (96543) | 96543..96959 | + | 417 | WP_001034044.1 | type II toxin-antitoxin system VapC family toxin | - |
| LHK10_RS24215 (97034) | 97034..98599 | + | 1566 | WP_001128474.1 | AAA family ATPase | - |
| LHK10_RS24220 (98584) | 98584..99606 | + | 1023 | WP_000361402.1 | helicase UvrD | - |
| LHK10_RS24225 (99860) | 99860..100546 | - | 687 | Protein_116 | IS1 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | blaCTX-M-15 / aac(6')-Ib-cr / blaOXA-1 / tet(A) / sitABCD | senB / iutA / iucD / iucC / iucB / iucA | 1..118805 | 118805 | |
| - | flank | IS/Tn | - | - | 99860..100207 | 347 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 102 a.a. Molecular weight: 11692.49 Da Isoelectric Point: 6.4674
>T220252 WP_001159871.1 NZ_CP085057:c95536-95231 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI
Download Length: 306 bp
>T220252 NZ_CP116994:c331694-331587 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA
Antitoxin
Download Length: 73 a.a. Molecular weight: 8354.27 Da Isoelectric Point: 4.5203
>AT220252 WP_000813630.1 NZ_CP085057:c95756-95538 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW
Download Length: 219 bp
>AT220252 NZ_CP116994:331741-331807 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829CCE8 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829CEF5 |