Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3476264..3476882 Replicon chromosome
Accession NZ_CP085056
Organism Escherichia coli strain P24_WM1_05.20

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LHK10_RS16920 Protein ID WP_001291435.1
Coordinates 3476664..3476882 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LHK10_RS16915 Protein ID WP_000344800.1
Coordinates 3476264..3476638 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LHK10_RS16905 (3471353) 3471353..3472546 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LHK10_RS16910 (3472569) 3472569..3475718 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
LHK10_RS16915 (3476264) 3476264..3476638 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LHK10_RS16920 (3476664) 3476664..3476882 + 219 WP_001291435.1 HHA domain-containing protein Toxin
LHK10_RS16925 (3477054) 3477054..3477605 + 552 WP_000102568.1 maltose O-acetyltransferase -
LHK10_RS16930 (3477721) 3477721..3478191 + 471 WP_000136192.1 YlaC family protein -
LHK10_RS16935 (3478355) 3478355..3479905 + 1551 WP_001372022.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LHK10_RS16940 (3479947) 3479947..3480300 - 354 WP_000878140.1 DUF1428 domain-containing protein -
LHK10_RS16950 (3480679) 3480679..3480990 + 312 WP_000409911.1 MGMT family protein -
LHK10_RS16955 (3481021) 3481021..3481593 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T220244 WP_001291435.1 NZ_CP085056:3476664-3476882 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T220244 NZ_CP116987:c4311911-4311738 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT220244 WP_000344800.1 NZ_CP085056:3476264-3476638 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT220244 NZ_CP116987:c4312235-4311939 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References