Detailed information of TA system TA220234

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1846605..1847437
• Replicon: chromosome
• Accession: NZ_CP085056
• Organism: Escherichia coli strain P24_WM1_05.20

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0Q2Y5N3
• Locus tag: LHK10_RS08855
• Protein ID: WP_001514886.1
• Coordinates: 1846605..1846979 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7U9IW59
• Locus tag: LHK10_RS08860
• Protein ID: WP_001360327.1
• Coordinates: 1847069..1847437 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LHK10_RS08820 (1842121)	1842121..1842594	+	474	WP_001105385.1	DNA gyrase inhibitor SbmC	-
LHK10_RS08825 (1842793)	1842793..1843851	+	1059	WP_032181141.1	FUSC family protein	-
LHK10_RS08830 (1844023)	1844023..1844352	+	330	WP_000450409.1	DUF496 family protein	-
LHK10_RS08835 (1844453)	1844453..1844719	-	267	WP_063121129.1	EutP/PduV family microcompartment system protein	-
LHK10_RS08840 (1845089)	1845089..1845460	-	372	WP_001295631.1	IS110 family transposase	-
LHK10_RS08845 (1846276)	1846276..1846356	-	81	Protein_1729	hypothetical protein	-
LHK10_RS08850 (1846456)	1846456..1846608	-	153	Protein_1730	DUF5983 family protein	-
LHK10_RS08855 (1846605)	1846605..1846979	-	375	WP_001514886.1	TA system toxin CbtA family protein	Toxin
LHK10_RS08860 (1847069)	1847069..1847437	-	369	WP_001360327.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LHK10_RS08865 (1847600)	1847600..1847821	-	222	WP_000692286.1	DUF987 domain-containing protein	-
LHK10_RS08870 (1847884)	1847884..1848360	-	477	WP_001186774.1	RadC family protein	-
LHK10_RS08875 (1848376)	1848376..1848849	-	474	WP_000855059.1	antirestriction protein	-
LHK10_RS08880 (1849191)	1849191..1850009	-	819	WP_088540043.1	DUF932 domain-containing protein	-
LHK10_RS08885 (1850127)	1850127..1850322	-	196	Protein_1737	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13930.91 Da
• Isoelectric Point: 7.2909

>T220234 WP_001514886.1 NZ_CP085056:c1846979-1846605 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T220234 NZ_CP116987:1825921-1826175 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13593.34 Da
• Isoelectric Point: 5.9598

>AT220234 WP_001360327.1 NZ_CP085056:c1847437-1847069 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT220234 NZ_CP116987:1825673-1825924 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A0Q2Y5N3

Antitoxin

• AlphaFold DB: A0A7U9IW59